College of Food Science, South China Agricultural Univ., 483 Wushan Street, Tianhe District, Guangzhou, 510642, China.
Dept. of Food Science, Rutgers Univ., 65 Dudley Road, New Brunswick, NJ, 08901, U.S.A.
J Food Sci. 2018 Feb;83(2):517-524. doi: 10.1111/1750-3841.14029. Epub 2018 Jan 16.
Zijuan tea (Camellia sinensis var. assamica) is a unique anthocyanin-rich tea cultivar in China. Although chemical component analysis of Zijuan tea and extraction technology of anthocyanins was widely documented, its functional properties have not been extensively explored. In this study, the anti-inflammatory, antiproliferative, and methylglyoxal (MGO) trapping activities of water extract (ZWE) and ethyl acetate extract (ZEE) of Zijuan tea were investigated for the 1st time. Results showed that ZWE and ZEE exhibited inhibitory effects on nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 production as well as inducible nitric oxide synthase protein (iNOS) expression in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Moreover, Zijuan tea extracts exerted stronger antiproliferative activity against HCT-116 cells compared with HepG2 and MDA-MB-231 cells, and thus could induce apoptosis in HCT-116 cells in a dose-dependent manner. Furthermore, Zijuan tea extracts were effective in trapping MGO under simulated physiological conditions, and the T (the time for 50% MGO remaining) values of ZWE and ZEE were 3.69 and 6.20 min, respectively. Additionally, the contents of total phenolics and catechins in ZEE were 685.43 ± 16.00 and 454.96 ± 4.21 mg/g extract, respectively, and in ZWE were 422.59 ± 12.09 and 307.29 ± 0.85 mg/g extract, respectively. Therefore, ZEE exhibited better anti-inflammatory, antiproliferative, and MGO trapping properties than ZWE may be mainly attributed to its higher (P < 0.05) content of total phenolics, expecially catechins. These results suggest that Zijuan tea could be a potential natural resource for the development of functional tea beverage.
This study revealed that Zijuan tea extracts possessed anti-inflammatory, antiproliferative, and methylglyoxal trapping potentials in vitro. With high anthocyanins and polyphenols, Zijuan tea can be developed into a healthy tea beverage or used as a natural component to reduce the level of methylglyoxal in Maillard reaction.
Zijuan 茶（Camellia sinensis var. assamica）是中国特有的一种富含花青素的茶品种。尽管 Zijuan 茶的化学成分分析和花青素提取技术已有广泛的文献记载，但它的功能特性尚未得到广泛的探索。本研究首次研究了 Zijuan 茶的水提取物（ZWE）和乙酸乙酯提取物（ZEE）的抗炎、抗增殖和甲基乙二醛（MGO）捕获活性。结果表明，ZWE 和 ZEE 对脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6 的产生以及诱导型一氧化氮合酶蛋白（iNOS）的表达具有抑制作用。此外，与 HepG2 和 MDA-MB-231 细胞相比，Zijuan 茶提取物对 HCT-116 细胞具有更强的抗增殖活性，并且能够以剂量依赖的方式诱导 HCT-116 细胞凋亡。此外，Zijuan 茶提取物在模拟生理条件下有效捕获 MGO，ZWE 和 ZEE 的 T（50% MGO 残留所需的时间）值分别为 3.69 和 6.20 分钟。此外，ZEE 中的总酚和儿茶素含量分别为 685.43±16.00 和 454.96±4.21mg/g 提取物，ZWE 中的总酚和儿茶素含量分别为 422.59±12.09 和 307.29±0.85mg/g 提取物。因此，ZEE 比 ZWE 表现出更好的抗炎、抗增殖和 MGO 捕获特性，可能主要归因于其较高的（P<0.05）总酚含量，特别是儿茶素。这些结果表明，Zijuan 茶可能是开发功能性茶饮料的潜在天然资源。
本研究表明，Zijuan 茶提取物在体外具有抗炎、抗增殖和甲基乙二醛捕获潜力。Zijuan 茶含有丰富的花青素和多酚，可开发成健康茶饮料，或用作天然成分，以降低美拉德反应中甲基乙二醛的水平。
