Inaba K, Koide S, Steinman R M
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7686-90. doi: 10.1073/pnas.82.22.7686.
During the primary mixed leukocyte reaction, T lymphocytes of the lyt-2- helper subclass proliferate in response to transplantation antigens on allogeneic dendritic cells. We have isolated populations of antigen-specific proliferating lymphoblasts and recultured them in fresh medium. Within 2 days, the blasts become smaller in size, lose responsiveness to T-cell growth factor or interleukin 2, but retain vigorous reactivity to the original alloantigen. Two new biologic properties of these "memory" lymphocytes have been noted. First, they primarily respond to alloantigen on dendritic cells, whereas freshly sensitized lymphoblasts react to allogeneic dendritic cells, macrophages, and B lymphocytes. Second, the memory lymphocytes quickly aggregate with dendritic cells that are either syngeneic or allogeneic, but not with B cells. The aggregates that form with syngeneic dendritic cells disassemble within hours and do not release interleukin 2 or proliferate. The aggregates that form with allogeneic dendritic cells remain intact, release large amounts of interleukin 2 on the first day of culture, and synthesize DNA on the second day. Therefore, dendritic cells actively cluster memory lymphocytes by an antigen-independent mechanism, and this may underlie the heightened functional activity of each cell type.
在初次混合淋巴细胞反应期间，lyt-2辅助亚类的T淋巴细胞会针对同种异体树突状细胞上的移植抗原发生增殖。我们已分离出抗原特异性增殖性淋巴母细胞群体，并将它们在新鲜培养基中再次培养。在2天内，这些母细胞体积变小，对T细胞生长因子或白细胞介素2失去反应性，但对原始同种异体抗原则保持强烈反应性。已注意到这些“记忆”淋巴细胞的两种新生物学特性。第一，它们主要对树突状细胞上的同种异体抗原作出反应，而新致敏的淋巴母细胞则对同种异体树突状细胞、巨噬细胞和B淋巴细胞作出反应。第二，记忆淋巴细胞会迅速与同基因或同种异体的树突状细胞聚集，但不与B细胞聚集。与同基因树突状细胞形成的聚集体在数小时内解体，不释放白细胞介素2也不增殖。与同种异体树突状细胞形成的聚集体保持完整，在培养的第一天释放大量白细胞介素2，并在第二天合成DNA。因此，树突状细胞通过一种不依赖抗原的机制主动聚集记忆淋巴细胞，这可能是每种细胞类型功能活性增强的基础。
