State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, and ‡State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan 430070, P. R. China.
ACS Appl Mater Interfaces. 2018 Feb 7;10(5):4369-4378. doi: 10.1021/acsami.7b13811. Epub 2018 Jan 26.
Development of novel antiviral reagents is of great importance for the control of virus spread. Here, AgS nanoclusters (NCs) were proved for the first time to possess highly efficient antiviral activity by using porcine epidemic diarrhea virus (PEDV) as a model of coronavirus. Analyses of virus titers showed that AgS NCs significantly suppressed the infection of PEDV by about 3 orders of magnitude at the noncytotoxic concentration at 12 h postinfection, which was further confirmed by the expression of viral proteins. Mechanism investigations indicated that AgS NCs treatment inhibits the synthesis of viral negative-strand RNA and viral budding. AgS NCs treatment was also found to positively regulate the generation of IFN-stimulating genes (ISGs) and the expression of proinflammation cytokines, which might prevent PEDV infection. This study suggest the novel underlying of AgS NCs as a promising therapeutic drug for coronavirus.
开发新型抗病毒试剂对于控制病毒传播非常重要。在这里，首次使用猪流行性腹泻病毒（PEDV）作为冠状病毒模型证明了 AgS 纳米团簇（NCs）具有高效的抗病毒活性。病毒滴度分析表明，AgS NCs 在感染后 12 小时以非细胞毒性浓度显著抑制 PEDV 的感染，约降低 3 个数量级，这通过病毒蛋白的表达进一步得到证实。机制研究表明，AgS NCs 处理抑制病毒负链 RNA 和病毒出芽的合成。还发现 AgS NCs 处理可正向调节 IFN 刺激基因（ISGs）和促炎细胞因子的产生，从而防止 PEDV 感染。本研究提示 AgS NCs 作为一种有前途的冠状病毒治疗药物具有新的作用机制。
