Paknejad Mojgan, Sattari Mandana, Akbari Solmaz, Mehrfard Ali, Aslroosta Hoori
Department of Periodontics, Dental Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Department of Immunology, Shahid Behashti University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2017 Dec;16(6):554-560.
The present study aimed to compare the levels of high-mobility group box 1(HMGB1) and soluble triggering receptor expressed on myeloid cells (sTREM1) in the gingival crevicular fluid (GCF). This cross-sectional cohort trial investigated two groups of 22 eligible chronic periodontitis and 22 periodontally healthy individuals (student volunteers) both before and after the periodontal treatment. GCF was collected from the deepest pockets with clinical attachment loss≥3 mm. Both groups received oral hygiene instructions, and scaling and root planning were performed in the test group. Enzyme-linked immunosorbent assay kit (ELISA) was used to measure the levels of HMGB1 and sTREM1 in GCF samples collected before and 1 month after non-surgical periodontal treatment. The results showed that HMGB1 levels were significantly higher in the chronic periodontitis patients than those of the healthy individuals before treatment (p<0.02) and decreased significantly after periodontal treatment, which reduced gingival inflammation. Furthermore, the levels of sTREM1 marker were significantly higher in periodontitis patients before (p<0.001) and 1 month after treatment than in healthy individuals (p<0.003) although its crevicular levels decreased after periodontal therapy in periodontitis group. The higher levels of sTREM1 and HMGB1 cytokines in GCF of periodontitis patients and the significant decrease after the introduction of the periodontal treatment underlines the importance of HMGB1 and sTREM1 in pathogenesis of periodontitis.
本研究旨在比较龈沟液（GCF）中高迁移率族蛋白B1（HMGB1）和髓系细胞表面可溶性触发受体（sTREM1）的水平。这项横断面队列试验调查了两组符合条件的人群，分别为22例慢性牙周炎患者和22例牙周健康个体（学生志愿者），在牙周治疗前后均进行了检测。从临床附着丧失≥3 mm的最深牙周袋中采集龈沟液。两组均接受了口腔卫生指导，试验组进行了龈上洁治和根面平整。采用酶联免疫吸附测定试剂盒（ELISA）检测非手术牙周治疗前及治疗后1个月采集的龈沟液样本中HMGB1和sTREM1的水平。结果显示，慢性牙周炎患者治疗前的HMGB1水平显著高于健康个体（p<0.02），牙周治疗后显著降低，牙龈炎症减轻。此外，牙周炎患者治疗前（p<0.001）和治疗后1个月的sTREM1标志物水平均显著高于健康个体（p<0.003），尽管牙周炎组牙周治疗后龈沟液中该标志物水平有所下降。牙周炎患者龈沟液中sTREM1和HMGB1细胞因子水平较高，牙周治疗后显著降低，这突出了HMGB1和sTREM1在牙周炎发病机制中的重要性。
