Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Centre, Peking Union Medical Collage (PUMC), No. 5 Panjiayuan Nanli, Chaoyang District, Beijing 10021, PR China.
Bioconjug Chem. 2018 Feb 21;29(2):250-254. doi: 10.1021/acs.bioconjchem.8b00023. Epub 2018 Jan 18.
Pretargeted immuno-PET imaging based on the bioorthogonal chemistry between F-labeled Reppe anhydride derivatives and tetrazine conjugates of the EGFR-specific monoclonal antibodies cetuximab and panitumumab was performed. This pretargeting approach yielded high target-to-nontarget ratios. Furthermore, due to the fast clearance rate of the PET probe, the overall radiation burden to nontarget tissues was also substantially decreased.
基于 F 标记的 Reppe 酐衍生物与 EGFR 特异性单克隆抗体西妥昔单抗和帕尼单抗的四嗪缀合物之间的生物正交化学，进行了靶向免疫 PET 成像。这种前靶向方法产生了高的靶与非靶比值。此外，由于 PET 探针的快速清除率，非靶组织的整体辐射负担也大大降低。
