Mutwale Kapepula Paulin, Baku Ngumbi Pacha, Kasongo Kawayidiko Michel, Duki Mpanzu Arthur, Franck Thierry, Mouithys-Mickalad Ange, Frédérich Michel, Ngombe Kabamba Nadege, Masiala Tsobo Christophe
a Faculty of Pharmaceutical Sciences, Centre d'Etudes des Substances Naturelles d'Origine Végétale (CESNOV) , University of Kinshasa , Kinshasa , Democratic Republic of Congo.
b Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM) , University of Liège , Liège , Belgium.
Nat Prod Res. 2019 Jun;33(11):1650-1654. doi: 10.1080/14786419.2018.1425847. Epub 2018 Jan 16.
, a traditional vegetable from Kongo Central area (DR. Congo) was studied for establishing microscopic characters and characterised by chromatographic techniques and their biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Microscopically leaf can be characterised by non-glandular and glandular trichomes, sinuous anticlinal epidermal cells, diacytic stomata and helical vessels. Methanolic extract displayed high cellular antioxidant activity at the concentrations range of 0.1-10 μg mL and 1-20 μg mL using lucigenin on neutrophils and DCFH-DA on HL 60, respectively. This extract also showed, more efficient effects on extracellular and intracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of were significantly higher, positively correlated with their phytochemical constituents such as flavonoids, iridoids and phenolic acids; and could justify their use as traditional vegetable and potent local nutraceutical resource.
对一种来自刚果民主共和国中刚果地区的传统蔬菜进行了研究，以确定其微观特征，并通过色谱技术对其进行表征，还在细胞模型中以及针对一种参与炎症反应的酶——髓过氧化物酶（MPO），评估了其对活性氧生成的生化活性。在显微镜下，叶片的特征为非腺毛和腺毛、波状的垂周表皮细胞、双细胞型气孔和螺旋状导管。甲醇提取物分别在0.1 - 10μg/mL和1 - 20μg/mL的浓度范围内，使用光泽精对中性粒细胞以及2′,7′-二氯二氢荧光素二乙酸酯（DCFH-DA）对HL-60细胞显示出较高的细胞抗氧化活性。该提取物还对细胞外和细胞内活性氧的生成以及MPO活性表现出更有效的作用。该蔬菜的抗氧化和抗炎活性显著更高，与黄酮类、环烯醚萜类和酚酸等植物化学成分呈正相关；这可以证明其作为传统蔬菜和有效的当地营养保健品资源的用途。
