School of Chemical Science and Engineering, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, PR China.
Department of Ophthalmology, Anhui Provincial Hospital, Hefei 230001, PR China.
Nanomedicine (Lond). 2018 Feb;13(3):339-351. doi: 10.2217/nnm-2017-0290. Epub 2018 Jan 17.
The combined efficacy of CuO nanoparticles (NPs) with 22 kinds of antibiotics against Escherichia coli was systematic studied, and CuO with cephalexin synergistic system was screened out.
Antimicrobial susceptibility test included disk diffusion test, checkerboard method and time-kill assay. The interactions of CuO NPs and antibiotics were analyzed by x-ray photoelectron spectroscopy, Fourier transform infrared spectra and Zeta. The interactions between bacteria and antibacterial agents were studied by surface plasmon resonance sensor for the first time.
RESULTS & CONCLUSION: Synergistic effect (1+1>2) was observed when CuO NPs combined with cephalexin against E. coli. The concentrated cephalexin molecules interacted more strongly with the E. coli cells to make cell wall become loose. Then, CuO NPs were more easily to damage and penetrate cells. Besides, the presence of antibiotics did not enhance Cu release, Cu uptake and reactive oxygen species generation. But the presence of cephalexin greatly enhanced cell permeability in comparison to others.
系统研究氧化铜纳米颗粒（NPs）与 22 种抗生素联合对大肠杆菌的疗效，并筛选出与头孢氨苄协同作用的氧化铜体系。
采用药敏试验（包括纸片扩散试验、棋盘试验和时间杀伤试验）来评估联合用药效果。采用 X 射线光电子能谱、傅里叶变换红外光谱和 Zeta 分析氧化铜 NPs 和抗生素之间的相互作用。首次利用表面等离子体共振传感器研究了细菌与抗菌药物之间的相互作用。
研究发现，氧化铜 NPs 与头孢氨苄联合使用对大肠杆菌具有协同作用（1+1>2）。浓缩的头孢氨苄分子与大肠杆菌细胞的相互作用更强，使细胞壁变得松散。然后，氧化铜 NPs 更容易破坏和穿透细胞。此外，抗生素的存在并没有增强 Cu 的释放、摄取和活性氧的产生。但与其他抗生素相比，头孢氨苄的存在大大增强了细胞通透性。
