Plishka R J, Cangro C B, Neale J H
Brain Res. 1985 Dec 23;360(1-2):403-6. doi: 10.1016/0006-8993(85)91264-8.
Glycylglutamine, the carboxyterminal sequence of beta-endorphin1-31, is produced as a free dipeptide during the posttranslational synthesis of beta-endorphin1-27. Antisera which recognize glycylglutamine were raised in rabbits and used for immunohistochemistry. With these antisera, glycylglutamine immunoreactivity was demonstrated in cells of the rat intermediate pituitary. In contrast, anterior pituitary cells, which exhibited beta-endorphin immunoreactivity, did not react with the anti-glycylglutamine sera. The conclusion that the antisera distinguished glycylglutamine immunoreactivity from beta-endorphin1-31 immunoreactivity is based upon cellular specificity, fixation requirements and blocking studies. The antisera demonstrated the differential expression of this dipeptide product of the proopiomelanocortin prohormone. The efficacy of carbodiimide as an immunohistochemical fixative for small molecules is also shown.
甘氨酰谷氨酰胺是β-内啡肽1-31的羧基末端序列，在β-内啡肽1-27的翻译后合成过程中作为游离二肽产生。针对甘氨酰谷氨酰胺的抗血清在兔体内产生，并用于免疫组织化学。利用这些抗血清，在大鼠垂体中间叶细胞中证实了甘氨酰谷氨酰胺免疫反应性。相比之下，表现出β-内啡肽免疫反应性的垂体前叶细胞与抗甘氨酰谷氨酰胺血清不发生反应。抗血清能够区分甘氨酰谷氨酰胺免疫反应性和β-内啡肽1-31免疫反应性这一结论是基于细胞特异性、固定要求和封闭研究得出的。抗血清证实了促阿片黑素皮质素原激素的这种二肽产物的差异表达。还展示了碳二亚胺作为小分子免疫组织化学固定剂的有效性。
