Beijing Key Laboratory for Agricultural Applications and New Techniques, Plant Science and Technology College, Beijing University of Agriculture , Beijing , China.
College of Biotechnology, Beijing University of Agriculture , Beijing , China.
Physiol Genomics. 2018 Mar 1;50(3):197-207. doi: 10.1152/physiolgenomics.00079.2017. Epub 2017 Dec 20.
studies have demonstrated that jasmonic acid (JA) reduces root-knot nematode (RKN) infections in tomato plants. RKN invasion is sensed by roots, and root-derived JA signaling activates systemic defense responses, though this is poorly understood. Here, we investigate variations in the RKN-induced transcriptome in scion phloem between two tomato plant grafts: CM/CM ( Lycopersicum esculentum Mill. cv. Castlemart) and CM/ spr2 (a JA-deficient mutant). A total of 8,716 genes were differentially expressed in the scion phloem of the plants with JA-deficient rootstock via RNA sequencing. Among these genes, 535 upregulated and 153 downregulated genes with high copy numbers were identified as significantly differentially expressed. Among them, 34 predicted transcription factor genes were identified. Additionally, we used real-time quantitative PCR to analyze the expression patterns of 42 genes involved in the JA, ethylene, or salicylic acid pathway in phloem under RKN infection. The results suggested that in the absence of JA signaling, the ET signaling pathway is enhanced after RKN infection; however, alterations in the SA signaling pathway were not observed.
研究表明，茉莉酸（JA）可减少番茄植株根结线虫（RKN）的感染。RKN 的入侵被根部感知，而根衍生的 JA 信号激活系统防御反应，但这一点知之甚少。在这里，我们研究了在两个番茄嫁接植物的接穗韧皮部中，RKN 诱导的转录组的变化：CM/CM（Lycopersicum esculentum Mill. cv. Castlemart）和 CM/spr2（JA 缺陷突变体）。通过 RNA 测序，在 JA 缺陷根砧植物的接穗韧皮部中，共鉴定出 8716 个差异表达基因。在这些基因中，有 535 个上调和 153 个下调的基因具有高拷贝数，被鉴定为显著差异表达。其中，鉴定出 34 个预测的转录因子基因。此外，我们使用实时定量 PCR 分析了在 RKN 感染下韧皮部中参与 JA、乙烯或水杨酸途径的 42 个基因的表达模式。结果表明，在缺乏 JA 信号的情况下，RKN 感染后 ET 信号通路增强；然而，SA 信号通路的改变并未观察到。
