Jones B M
Clin Exp Immunol. 1985 Sep;61(3):642-7.
Pokeweed mitogen (PWM)-induced B-cell activation was entirely abrogated by pre-treating peripheral mononuclear cells (PBM) with T4 (anti-T-helper-inducer subset) or T11 (anti-sheep erythrocyte receptor, ie. anti-pan-T-cell) monoclonal antibody plus complement. Immunoglobulin secretion was restored in T11- but not T4- cultures by adding the culture supernatant from PWM-stimulated PBM (T-replacing factor, TRF). Since T8+ (T-suppressor-cytotoxic subset) cells were present in high concentrations in T4- but absent from T11- cultures, it appeared that these cells could inhibit B-cell activation by PWM plus TRF and that suppression could occur in the absence of T4+ cells. Incubation of TRF with purified, unstimulated T8+ cells prior to addition to T11- cultures abrogated subsequent PWM-induced B-cell activation, whereas T8- PBM had no effect on TRF. T8+ cells did not secrete suppressor factors during incubation with TRF. Absorption of one or more of the factors required for B-cell growth and differentiation by T8+ cells appears to be an important suppressor mechanism operating in the PWM system.
用T4（抗辅助性T诱导亚群）或T11（抗绵羊红细胞受体，即抗全T细胞）单克隆抗体加补体预处理外周血单个核细胞（PBM），可完全消除商陆有丝分裂原（PWM）诱导的B细胞活化。通过添加PWM刺激的PBM培养上清液（T替代因子，TRF），T11处理的培养物中免疫球蛋白分泌得以恢复，而T4处理的培养物中则未恢复。由于T8 +（抑制性细胞毒性亚群）细胞在T4处理的培养物中浓度较高，而在T11处理的培养物中不存在，因此似乎这些细胞可以抑制PWM加TRF诱导的B细胞活化，并且在没有T4 +细胞的情况下也可能发生抑制作用。在添加到T11处理的培养物之前，将TRF与纯化的、未刺激的T8 +细胞一起孵育，可消除随后PWM诱导的B细胞活化，而T8 - PBM对TRF没有影响。T8 +细胞在与TRF孵育期间不分泌抑制因子。T8 +细胞吸收B细胞生长和分化所需的一种或多种因子似乎是PWM系统中起作用的一种重要抑制机制。
