Jones B M
Scand J Immunol. 1983 Nov;18(5):399-406. doi: 10.1111/j.1365-3083.1983.tb00871.x.
Peripheral blood mononuclear cells (PBMN) that were depleted of OKT8+ cells and stimulated with pokeweed mitogen (PWM) produced higher cell yields and higher numbers of plaque-forming cells than unfractionated PBMN. Conversely, OKT8-enriched PBMN, prepared by mixing unfractionated and OKT8+ cells in a ratio of 3:1, gave reduced cell growth and B-cell activation. In OKT8-depleted cultures, B cells, OKT4+ cells, OKT8+ cells, and OKM1+ cells increased in number between days 4 and 7 of culture by factors of 9.8, 5.9, 20.1, and 5.6 respectively, whereas growth rates for these subsets were 2.4, 1.0, 2.0, and 1.3 in unfractionated cultures and 0.9, 1.0, 1.2, and 0.6 in cultures enriched for OKT8+ cells. On day 7 of culture, 73 +/- 10% of B cells secreted immunoglobulin in unfractionated cultures, whereas only 21 +/- 10% of B cells were activated in OKT8-enriched cultures. Surprisingly, PWM stimulation of OKT8-depleted PBMN produced only 40 +/- 12% activated B cells.
去除OKT8+细胞并用商陆丝裂原（PWM）刺激的外周血单个核细胞（PBMN）比未分离的PBMN产生更高的细胞产量和更多的噬斑形成细胞。相反，通过将未分离的细胞与OKT8+细胞按3:1的比例混合制备的富含OKT8的PBMN，其细胞生长和B细胞活化降低。在去除OKT8的培养物中，B细胞、OKT4+细胞、OKT8+细胞和OKM1+细胞在培养的第4天至第7天数量分别增加了9.8、5.9、20.1和5.6倍，而在未分离的培养物中这些亚群的生长速率分别为2.4、1.0、2.0和1.3，在富含OKT8+细胞的培养物中分别为0.9、1.0、1.2和0.6。在培养的第7天，未分离的培养物中73±10%的B细胞分泌免疫球蛋白，而在富含OKT8的培养物中只有21±10%的B细胞被激活。令人惊讶的是，PWM刺激去除OKT8的PBMN仅产生40±12%的活化B细胞。
