Li Jingjing, Wang Junhui, Sun Dong, Dai Yue, Shen Jiahui, You Jia, Han Cuiping, Xu Kai
J Biomed Nanotechnol. 2016 Aug;12(8):1604-616. doi: 10.1166/jbn.2016.2271.
Magnetic resonance imaging (MRI) with high spatial resolution has been widely used in clinics as a noninvasive diagnostic technology, and MRI diagnosis-based theranostic nanomaterials have attracted increasing attention. Herein, we report on the fabrication of a GO/BSA-Gd2O3/AS1411-DOX theranostic nanocomplex with BSA-Gd2O3 nanoparticles for use as an MRI contrast agent (CA), with graphene oxide (GO) nanoplates as the CA and drug nanocarrier, as well as an aptamer, AS1411, as the targeting molecule. The proposed theranostic nanocomplex not only provided stronger MR contrast enhancement but also inhibited the growth of 786-0 human renal carcinoma cells with the help of the AS1411 aptamer, while sparing the normal cells from harm, thus demonstrating their specific drug delivery capability. Additionally, 786-0 cells could be specifically recognized using the GO/BSA-Gd2O3/AS1411-DOX theranostic nanocomplex with MRI both in vitro and in vivo. Notably, most of the nanocomplex that was injected into the tail vein was excreted by the kidneys and bladder, while MRI signals from the nanocomplexes that accumulated specifically in the tumor region could remain as long as 24 hours, which is beneficial for future clinical diagnosis and therapy.
具有高空间分辨率的磁共振成像（MRI）作为一种非侵入性诊断技术已在临床上广泛应用，基于MRI诊断的诊疗纳米材料也越来越受到关注。在此，我们报道了一种GO/BSA-Gd2O3/AS1411-DOX诊疗纳米复合物的制备，该复合物以BSA-Gd2O3纳米颗粒作为MRI造影剂（CA），氧化石墨烯（GO）纳米片作为造影剂和药物纳米载体，以及适配体AS1411作为靶向分子。所提出的诊疗纳米复合物不仅提供了更强的磁共振对比增强效果，还借助AS1411适配体抑制了786-0人肾癌细胞的生长，同时使正常细胞免受伤害，从而证明了它们的特异性药物递送能力。此外，GO/BSA-Gd2O3/AS1411-DOX诊疗纳米复合物在体外和体内均可通过MRI特异性识别786-0细胞。值得注意的是，注入尾静脉的大部分纳米复合物通过肾脏和膀胱排出，而特异性积聚在肿瘤区域的纳米复合物的MRI信号可以保留长达24小时，这对未来的临床诊断和治疗有益。
