Department of Medical Genetics, China Medical University, Shenyang 110122, China.
Teaching and Experiment Center, Liaoning University of Traditional Chinese Medicine, Shenyang110847, China.
Int J Mol Sci. 2018 Jan 13;19(1):236. doi: 10.3390/ijms19010236.
is a downstream gene of . miR-3189 is embedded in the intron of -and coexpressed with it. miR-3189-3p functions to inhibit the proliferation and migration of glioblastoma cells. We speculated that might regulate miR-3189-3p to affect its function in gastric cancer cells. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed that miR-3189-3p expression was significantly downregulated in MGC803 cells after knockdown. Overexpression of miR-3189-3p significantly inhibited the proliferation and migration of the cells. Moreover, miR-3189-3p mimics enhanced the effects of an siRNA on the inhibition of cell proliferation and migration. Dual luciferase reporter assays, qRT-PCR, and Western blotting verified that is a direct target of miR-3189-3p. mediates the regulation of miR-3189-3p on the proliferation and migration of MGC803 cells. Data mining based on Kaplan-Meier plots showed that high expression is associated with poor overall survival and first progression in gastric cancer. These data suggested that miR-3189-3p mimics enhanced the effects of the siRNA on the inhibition of gastric cancer cell proliferation and migration by targeting . The findings suggested that when targeting to treat gastric cancer, consideration and correction for counteracting factors should obtain a satisfactory effect.
是下游基因。miR-3189 嵌入 - 的内含子中，并与其共表达。miR-3189-3p 可抑制神经胶质瘤细胞的增殖和迁移。我们推测可能通过调节 miR-3189-3p 来影响其在胃癌细胞中的功能。实时定量逆转录聚合酶链反应 (qRT-PCR) 显示，敲低后 MGC803 细胞中 miR-3189-3p 的表达明显下调。miR-3189-3p 的过表达显著抑制了细胞的增殖和迁移。此外，miR-3189-3p 模拟物增强了 an siRNA 对细胞增殖和迁移抑制的作用。双荧光素酶报告基因检测、qRT-PCR 和 Western blot 验证了是 miR-3189-3p 的直接靶基因。调节 miR-3189-3p 对 MGC803 细胞增殖和迁移的作用。基于 Kaplan-Meier 图的数据挖掘表明，高表达与胃癌患者总生存期和首次进展不良相关。这些数据表明，miR-3189-3p 模拟物通过靶向增强了 an siRNA 对胃癌细胞增殖和迁移的抑制作用。这些发现表明，在针对治疗胃癌时，应考虑和纠正对抗因素，以获得满意的效果。
