Lee Sun Haeng, Lee Hyun Jeong, Lee Sung Hyun, Kim Young-Sik, Lee Donghun, Chun Jiu, Lee Jin Yong, Kim Hocheol, Chang Gyu Tae
1Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul, 02447 Republic of Korea.
2Department of Pediatrics of Korean Medicine, Kyung Hee University Korean Medicine Hospital, Kyung Hee University Medical Center, Seoul, 02447 Republic of Korea.
Chin Med. 2018 Jan 12;13:3. doi: 10.1186/s13020-017-0156-7. eCollection 2018.
To evaluate the effects of Huang Bai () on growth and maturation in adolescent female rats.
Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg). Body weight, food intake, and vaginal opening were measured daily from postnatal day (PND) 28 to PND 43. Tetracycline (20 mg/kg) was injected on PND 41. After sacrifice on PND 43, the ovaries and uterus were weighed, and the tibias were fixed in 4% paraformaldehyde. Decalcified and dehydrated tibias were sectioned at a thickness of 40 μm, and sectioned tissues were examined with a fluorescence microscope. Insulin-like growth factor (IGF)-1 and bone morphogenetic protein (BMP)-2 were detected using immunohistochemistry.
Relative to controls, body weight was higher in the triptorelin group. Bone growth rate increased in the Huang Bai 100 mg/kg (354.00 ± 31.1 μm/day), rhGH (367.10 ± 27.11 μm/day), and triptorelin (374.50 ± 25.37 μm/day) groups. Expression of IGF-1 and BMP-2 in the hypertrophic zone was higher in all experimental groups. Vaginal opening occurred earlier in the estradiol group (PND 33.58 ± 1.62) than in controls and later in the triptorelin group (PND > 43). Ovarian and uterine weights were lower in the oestradiol and triptorelin groups. However, Huang Bai had nonsignificant effects on vaginal opening and the weights of ovaries and the uterus.
Huang Bai stimulated bone growth by upregulating IGF-1 and BMP-2 in the growth plate. However, it had no effect on pubertal development.
评估黄柏对青春期雌性大鼠生长和成熟的影响。
将雌性斯普拉格-道利大鼠（28日龄；n = 72）分为六个每日治疗组：对照组（蒸馏水）、黄柏（100和300毫克/千克）、重组人生长激素（rhGH；20微克/千克）、雌二醇（1微克/千克）和曲普瑞林（100微克）。从出生后第28天（PND）至PND 43每天测量体重、食物摄入量和阴道开口情况。在PND 41注射四环素（20毫克/千克）。在PND 43处死后，称量卵巢和子宫重量，将胫骨固定于4%多聚甲醛中。脱钙并脱水后的胫骨切成40微米厚的切片，用荧光显微镜检查切片组织。采用免疫组织化学法检测胰岛素样生长因子（IGF）-1和骨形态发生蛋白（BMP）-2。
与对照组相比，曲普瑞林组体重更高。黄柏100毫克/千克组（354.00±31.1微米/天）、rhGH组（367.10±27.11微米/天）和曲普瑞林组（374.50±25.37微米/天）的骨生长速率增加。所有实验组肥大区IGF-1和BMP-2的表达均较高。雌二醇组阴道开口时间早于对照组（PND 33.58±1.62），曲普瑞林组则晚于对照组（PND>43）。雌二醇组和曲普瑞林组的卵巢和子宫重量较低。然而，黄柏对阴道开口以及卵巢和子宫重量无显著影响。
黄柏通过上调生长板中的IGF-1和BMP-2促进骨生长。然而它对青春期发育没有影响。
