Bai Jun-Wen, Wang Xia, Zhang Ya-Feng, Yao Guo-Dong, Liu Hong
The Second Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
Oncol Lett. 2017 Dec;14(6):7145-7152. doi: 10.3892/ol.2017.7087. Epub 2017 Sep 27.
Dysregulation of microRNAs (miRs) can contribute to cancer development and progression. In the present study, the function and underlying molecular mechanisms of miR-320 in breast cancer tumorigenesis and progression were investigated. The results of a reverse transcription-quantitative polymerase chain reaction analysis demonstrated that miR-320 was frequently downregulated in breast cancer tissues compared with adjacent normal tissues. In addition, knockdown of miR-320 in breast cancer cell lines promoted cell proliferation and invasion , whereas miR-320 overexpression had the opposite effect. Furthermore, a Dual-Luciferase reporter assay indicated that SRY-box 4 (SOX4) is a direct target of miR-320, and the restoration of SOX4 in miR-320-overexpressing cells attenuated the tumor-suppressive effects of miR-320. Collectively, these results indicated that miR-320 acts as a tumor suppressor in breast cancer tumorigenesis and progression.
微小RNA（miR）失调可促进癌症的发生和发展。在本研究中，对miR-320在乳腺癌发生和发展中的功能及潜在分子机制进行了研究。逆转录-定量聚合酶链反应分析结果表明，与相邻正常组织相比，miR-320在乳腺癌组织中经常下调。此外，在乳腺癌细胞系中敲低miR-320可促进细胞增殖和侵袭，而miR-320过表达则产生相反的效果。此外，双荧光素酶报告基因检测表明，SRY盒4（SOX4）是miR-320的直接靶点，在miR-320过表达细胞中恢复SOX4可减弱miR-320的肿瘤抑制作用。总体而言，这些结果表明miR-320在乳腺癌发生和发展中起肿瘤抑制作用。
