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通过结合mRNA和miRNA表达谱对耐药性小细胞肺癌细胞系进行基因组分析。

Genomic analysis of drug resistant small cell lung cancer cell lines by combining mRNA and miRNA expression profiling.

作者信息

Chen Yitian, Yang Xiang, Xu Yichen, Cao Jiongrui, Chen Longbang

机构信息

Department of Medical Oncology, Jinling Hospital, Second Military Medical University, Nanjing, Jiangsu 210002, P.R. China.

Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, P.R. China.

出版信息

Oncol Lett. 2017 Jun;13(6):4077-4084. doi: 10.3892/ol.2017.5967. Epub 2017 Mar 31.

DOI:10.3892/ol.2017.5967
PMID:28599409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5452998/
Abstract

Etoposide (VP16) combined with cisplatin (DDP), as the first-line chemotherapy for small cell lung cancer (SCLC), regularly confers drug resistance. The present study applied complementary (c)DNA and micro (mi)RNA microarray to identify gene and miRNA expression profiles associated with multidrug resistance (MDR) in SCLC. The VP16/DDP (VP16 combined with DDP) resistant SCLC H446/EP cell line was derived from the parental H446 cell line by continuous exposure to increasing concentrations of etoposide and cisplatin. The mRNA and miRNA expression profiles between the resistant and parental SCLC cells were analyzed by Phalanx OneArray™ mRNA and miRNA microarray, and the results were confirmed by quantitative polymerase chain reaction. The expression levels of 75 genes were downregulated whilst 40 genes were upregulated in the H446/EP cell line compared with the H446 cell line. The expression levels of 16 miRNAs were upregulated whilst 15 were downregulated in the H446/EP cell line compared with the H446 cell line. Expression profile studies indicate that the particular mRNA and miRNA alteration demonstrated in MDR of SCLC may provide potential biomolecular targets for MDR reversion.

摘要

依托泊苷（VP16）联合顺铂（DDP）作为小细胞肺癌（SCLC）的一线化疗方案，常出现耐药情况。本研究应用互补（c）DNA和微小（mi）RNA微阵列来鉴定与SCLC多药耐药（MDR）相关的基因和miRNA表达谱。VP16/DDP（VP16联合DDP）耐药的SCLC H446/EP细胞系是通过将亲本H446细胞系持续暴露于浓度递增的依托泊苷和顺铂而获得的。采用Phalanx OneArray™ mRNA和miRNA微阵列分析耐药和亲本SCLC细胞之间的mRNA和miRNA表达谱，并通过定量聚合酶链反应对结果进行验证。与H446细胞系相比，H446/EP细胞系中有75个基因的表达水平下调，40个基因的表达水平上调。与H446细胞系相比，H446/EP细胞系中有16个miRNA的表达水平上调，15个miRNA的表达水平下调。表达谱研究表明，SCLC多药耐药中表现出的特定mRNA和miRNA改变可能为多药耐药逆转提供潜在的生物分子靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/d90c51bf70d9/ol-13-06-4077-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/e960858ca183/ol-13-06-4077-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/be9c573c2d37/ol-13-06-4077-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/a08351a2605a/ol-13-06-4077-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/d90c51bf70d9/ol-13-06-4077-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/e960858ca183/ol-13-06-4077-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/be9c573c2d37/ol-13-06-4077-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/a08351a2605a/ol-13-06-4077-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d05f/5452998/d90c51bf70d9/ol-13-06-4077-g03.jpg

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