Yang Yang, Liang Shengru, Li Yuqian, Gao Fei, Zheng Longlong, Tian Shilai, Yang Pu, Li Lihong
Department of Neurosurgery, The 451st Hospital of Chinese People's Liberation Army, Xi'an, Shaanxi 710054, P.R. China.
Department of Gynaecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
Oncol Lett. 2017 Dec;14(6):7179-7184. doi: 10.3892/ol.2017.7180. Epub 2017 Oct 13.
Hepatoma-derived growth factor (HDGF) regulates various cellular processes involved in the onset and development of tumors. To evaluate the role of HDGF in human gliomas, western blotting analysis, immunohistochemistry staining and reverse transcription-quantitative polymerase chain reaction were performed to detect HDGF protein and mRNA expression levels in glioma and intractable epileptic brain tissue. Various clinicopathological characteristics, including age, gender, World health Organization grade, HDGF expression level, Karnofsky performance Status (KPS) and Ki-67 index were obtained from medical records. The correlation between HDGF expression and these clinicopathological characteristics was statistically evaluated. Following this, multivariate liner regression was used to evaluate their effect on patient survival time. HDGF expression, at the protein and mRNA levels, was observed to be more upregulated in glioma tissues compared with intractable epileptic brain tissue without tumor. Furthermore, the level of HDGF expression was positively associated with the grade of malignancy [grades II~IV, Ki-67 index ≥20% or KPS <80 (P<0.05)] and poor prognosis in glioma patients. Notably, the univariate survival analysis identified a negative correlation between HDGF-expression and survival time (P<0.01) and multivariate liner regression demonstrated that HDGF expression is an independent prognostic factor for gliomas (P=0.01). Overall, HDGF upregulation may be a crucial step in the development and invasion of glioma. Further survival analysis highlighted its prognostic value for this malignancy, implying its potential as a promising therapeutic target for gliomas.
肝癌衍生生长因子（HDGF）调节肿瘤发生和发展过程中涉及的各种细胞过程。为了评估HDGF在人类胶质瘤中的作用，进行了蛋白质印迹分析、免疫组织化学染色和逆转录定量聚合酶链反应，以检测HDGF蛋白和mRNA在胶质瘤和难治性癫痫脑组织中的表达水平。从病历中获取了各种临床病理特征，包括年龄、性别、世界卫生组织分级、HDGF表达水平、卡诺夫斯基表现状态（KPS）和Ki-67指数。对HDGF表达与这些临床病理特征之间的相关性进行了统计学评估。在此之后，使用多元线性回归来评估它们对患者生存时间的影响。与无肿瘤的难治性癫痫脑组织相比，在胶质瘤组织中观察到HDGF在蛋白质和mRNA水平上的表达上调更为明显。此外，HDGF表达水平与恶性程度[II~IV级、Ki-67指数≥20%或KPS<80（P<0.05）]呈正相关，并且与胶质瘤患者的预后不良相关。值得注意的是，单因素生存分析确定HDGF表达与生存时间之间存在负相关（P<0.01），多元线性回归表明HDGF表达是胶质瘤的独立预后因素（P=0.01）。总体而言，HDGF上调可能是胶质瘤发生和侵袭的关键步骤。进一步的生存分析突出了其对这种恶性肿瘤的预后价值，这意味着它作为胶质瘤有前景的治疗靶点的潜力。
