Cong Fang, Yu Haitao, Gao Xiuhua
Department of General Surgery, Weihai Wendeng People's Hospital, Weihai, Shandong 264400, P.R. China.
Department of Clinical Laboratories, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China.
Oncol Lett. 2017 Dec;14(6):7185-7190. doi: 10.3892/ol.2017.7142. Epub 2017 Oct 5.
This study aimed to investigate the correlation between the expression of CD24 and B7-H3 in breast cancer tissues and the clinical significance. Expression of CD24 and B7-H3 in breast cancer and adjacent tissues were detected by immunohistochemistry. Quantitative PCR was used to detect the expression of CD24 and B7-H3 mRNA in breast cancer and adjacent tissues. The expression of CD24 and B7-H3 protein in breast cancer and adjacent tissues was detected by immunoblotting. The correlation between the expression levels of the two proteins was analyzed and the relationship between the expression of two proteins and the 5-year survival of breast cancer patients was investigated. CD24 and B7-H3 were positively expressed in breast cancer and adjacent tissues, the CD24-positive rate was 75.7 and 25.7%, respectively, and the B7-H3-positive rate was 56.8 and 43.2%, respectively, and the differences were statistically significant (P<0.05). The expression of CD24 was positively correlated with the expression of B7-H3 (Spearman's correlation coefficient r, 0.297; p=0.036). The positive and negative expression of CD24 and B7-H3 significantly affected the 5-year survival of breast cancer patients (P<0.05). Quantitative PCR results showed that the expression levels of CD24 and B7-H3 mRNA in breast cancer tissues were significantly higher than those in adjacent tissues (P<0.05). The expression levels of CD24 and B7-H3 protein in breast cancer tissues were also significantly higher than those in adjacent tissues (P<0.05). CD24 and B7-H3 were highly expressed in breast cancer, suggesting that both CD24 and B7-H3 were related to the development of breast cancer. Five-year survival analysis of breast cancer patients showed that the high expression of CD24 and B7-H3 were correlated with the poor prognosis of patients. Thus, CD24 and B7-H3 may become new targets for the treatment of breast cancer.
本研究旨在探讨乳腺癌组织中CD24和B7-H3的表达相关性及其临床意义。采用免疫组织化学法检测乳腺癌组织及癌旁组织中CD24和B7-H3的表达。运用定量PCR检测乳腺癌组织及癌旁组织中CD24和B7-H3 mRNA的表达。通过免疫印迹法检测乳腺癌组织及癌旁组织中CD24和B7-H3蛋白的表达。分析两种蛋白表达水平之间的相关性，并研究两种蛋白表达与乳腺癌患者5年生存率的关系。CD24和B7-H3在乳腺癌组织及癌旁组织中均呈阳性表达，CD24阳性率分别为75.7%和25.7%，B7-H3阳性率分别为56.8%和43.2%，差异具有统计学意义（P<0.05）。CD24的表达与B7-H3的表达呈正相关（Spearman相关系数r为0.297；P=0.036）。CD24和B7-H3的阳性和阴性表达显著影响乳腺癌患者的5年生存率（P<0.05）。定量PCR结果显示，乳腺癌组织中CD24和B7-H3 mRNA的表达水平显著高于癌旁组织（P<0.05）。乳腺癌组织中CD24和B7-H3蛋白的表达水平也显著高于癌旁组织（P<0.05）。CD24和B7-H3在乳腺癌中高表达，提示CD24和B7-H3均与乳腺癌的发生发展有关。乳腺癌患者的5年生存分析表明，CD24和B7-H3的高表达与患者预后不良相关。因此，CD24和B7-H3可能成为乳腺癌治疗的新靶点。
