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miR-10b对鼻咽癌细胞上皮-间质转化、侵袭和迁移的调控机制研究

Study of miR-10b regulatory mechanism for epithelial-mesenchymal transition, invasion and migration in nasopharyngeal carcinoma cells.

作者信息

Wang Weiyi

机构信息

Department of Ear-Nose-Throat, Hunan Provincial People's Hospital, Changsha, Hunan 410005, P.R. China.

出版信息

Oncol Lett. 2017 Dec;14(6):7207-7210. doi: 10.3892/ol.2017.7172. Epub 2017 Oct 11.

DOI:10.3892/ol.2017.7172
PMID:29344154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5754829/
Abstract

The aim of the study was to investigate the miR-10b regulatory mechanism for epithelial-mesenchymal transition (EMT) and its effect on the proliferation and migration of nasopharyngeal carcinoma cells. RT-qPCR was used to detect the expression of miR-10b in CNE1 nasopharyngeal carcinoma cell line. The NP69 nasopharyngeal mucosal cell line was used to determine the expression of miR-10b after infection with lentivirus. The effect of miR-10b on the proliferation of NP69 was examined using cell counting kit-8. The effect of miR-10b on NP69 migration was examined using scratch assay. Western blot analysis was used to detect the effects of miR-10b on the expression of epithelial cell markers E-cadherin and β-catenin and mesenchymal cell markers fibronectin, N-cadherin, vimentin and matrix metalloproteinase-9 (MMP-9). The present study showed that miR-10b was highly expressed in CNE1 cells. The stable expression of miR-10b promoted the proliferation and migration of NP69 cells, downregulated the expression of epithelial cell markers E-cadherin and β-catenin, and upregulated the expression of mesenchymal cell markers fibronectin, N-cadherin, vimentin and MMP-9 resulting in cell EMT. In conclusion, miR-10b promotes the proliferation and migration of nasopharyngeal carcinoma cells, and induces EMT in nasopharyngeal carcinoma cells, thereby having the potential to become a new target for the treatment of nasopharyngeal carcinoma.

摘要

本研究旨在探讨miR-10b对上皮-间质转化（EMT）的调控机制及其对鼻咽癌细胞增殖和迁移的影响。采用RT-qPCR检测CNE1鼻咽癌细胞系中miR-10b的表达。利用NP69鼻咽黏膜细胞系检测慢病毒感染后miR-10b的表达。使用细胞计数试剂盒-8检测miR-10b对NP69细胞增殖的影响。采用划痕试验检测miR-10b对NP69细胞迁移的影响。通过蛋白质免疫印迹分析检测miR-10b对上皮细胞标志物E-钙黏蛋白和β-连环蛋白以及间充质细胞标志物纤连蛋白、N-钙黏蛋白、波形蛋白和基质金属蛋白酶-9（MMP-9）表达的影响。本研究表明，miR-10b在CNE1细胞中高表达。miR-10b的稳定表达促进了NP69细胞的增殖和迁移，下调了上皮细胞标志物E-钙黏蛋白和β-连环蛋白的表达，上调了间充质细胞标志物纤连蛋白、N-钙黏蛋白、波形蛋白和MMP-9的表达，导致细胞发生EMT。综上所述，miR-10b促进鼻咽癌细胞的增殖和迁移，并诱导鼻咽癌细胞发生EMT，从而有可能成为鼻咽癌治疗的新靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/dfc6a19cc481/ol-14-06-7207-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/e179c7b7192a/ol-14-06-7207-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/ed37262c17a6/ol-14-06-7207-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/ee27eb2b1b78/ol-14-06-7207-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/5e89fd638c35/ol-14-06-7207-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/dfc6a19cc481/ol-14-06-7207-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/e179c7b7192a/ol-14-06-7207-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/ed37262c17a6/ol-14-06-7207-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/ee27eb2b1b78/ol-14-06-7207-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/5e89fd638c35/ol-14-06-7207-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a22d/5754829/dfc6a19cc481/ol-14-06-7207-g04.jpg

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