Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400, Centro, São Carlos, SP, CEP 13560-970, Brazil.
Department of Physics from Federal University of Piauí (UFPI), Campus Universitário Ministro Petrônio Portella, Bairro Ininga, Teresina, Bairro Ininga, Teresina, PI 64049-550, Brazil.
J Bone Miner Metab. 2019 Jan;37(1):18-27. doi: 10.1007/s00774-018-0903-6. Epub 2018 Jan 17.
There are few published data on the relationship between loss of bone mass due to osteoporosis and poor tooth quality. This study analyzed the effects of osteoporosis on incisor teeth and femoral bones using optical techniques in rats. Twenty female Wistar rats aged 6 months (n = 20) were randomized into two groups: control group, non-ovariectomized rats (n = 10); ovariectomy group, ovariectomized rats to induce osteoporosis (n = 10). Each group was subdivided randomly into two groups containing five rats each as follows. Control group 1: non-ovariectomized rats euthanized at the age of 9 or 3 months post-ovariectomy (n = 5); Control group 2: non-ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (n = 5); ovariectomy group 1: ovariectomized rats euthanized at the age of 9 months or 3 months post-ovariectomy (n = 5); ovariectomy group 2: ovariectomized rats euthanized at the age of 1 year or 6 months post-ovariectomy (n = 5). The incisor teeth and femoral bones of Wistar rats were removed to perform Raman spectroscopy using an excitation laser at 785 nm. In addition, an energy-dispersive X-ray spectrometer system was used to evaluate calcium (Ca) and phosphorus (P). The main findings included significant changes (p < 0.05) for phosphate and carbonate band areas for both incisor teeth and femur bones. In addition, there was significant negative correlation between the P concentration and phosphate/carbonate ratio (lower P content-larger ratio, p < 0.05) for incisor teeth and femoral bones. The proline and CH wag band areas were significantly reduced only for the incisor teeth (p < 0.05). Therefore, Raman spectroscopy assessed the compositional, physicochemical and structural changes in hard tissue. The current study also pointed out the possible action mechanisms of these changes, bone fracture risk and dental fragility. It is important to emphasize that poor dental quality may also occur due to osteoporosis.
关于骨质疏松导致的骨量丢失与牙齿质量差之间的关系，目前仅有少量文献报道。本研究使用光学技术分析了骨质疏松对大鼠切牙和股骨的影响。选取 6 月龄雌性 Wistar 大鼠 20 只（n=20），随机分为两组：对照组，未行卵巢切除术的大鼠（n=10）；卵巢切除术组，行卵巢切除术诱导骨质疏松的大鼠（n=10）。每组再随机分为两组，每组 5 只。对照组 1：未行卵巢切除术的大鼠，于 9 月龄或卵巢切除术后 3 个月时安乐死（n=5）；对照组 2：未行卵巢切除术的大鼠，于 1 岁或卵巢切除术后 6 个月时安乐死（n=5）；卵巢切除术组 1：卵巢切除术组的大鼠，于 9 月龄或卵巢切除术后 3 个月时安乐死（n=5）；卵巢切除术组 2：卵巢切除术组的大鼠，于 1 岁或卵巢切除术后 6 个月时安乐死（n=5）。取出 Wistar 大鼠的切牙和股骨，使用 785nm 激发激光进行拉曼光谱分析。此外，还使用能量色散 X 射线光谱系统评估钙（Ca）和磷（P）。主要发现包括：切牙和股骨的磷酸盐和碳酸盐带面积均发生显著变化（p<0.05）。此外，切牙和股骨的 P 浓度与磷酸盐/碳酸盐比值呈显著负相关（P 含量越低，比值越大，p<0.05）。只有切牙的脯氨酸和 CH 摇摆带面积显著减少（p<0.05）。因此，拉曼光谱评估了硬组织的成分、物理化学和结构变化。本研究还指出了这些变化、骨折风险和牙齿脆弱性的可能作用机制。需要强调的是，骨质疏松也可能导致牙齿质量变差。
