Mahalingam Meera
Dermatopathology Section, Department of Pathology and Laboratory Medicine, VA Medical Center, West Roxbury, MA, USA.
Methods Mol Biol. 2018;1723:1-17. doi: 10.1007/978-1-4939-7558-7_1.
Laser capture microdissection is a non-molecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. Its advantages include efficient rapid and precise procurement of cells. The potential disadvantages include time consuming, expensive, and limited by the need for a pathologist for recognition of distinct subpopulations within a specified sample. Overall it is versatile allowing the preparation of homogenous isolates of specific subpopulations of cells from which DNA/RNA or protein can be extracted for RT-PCR, quantitative PCR, next-generation sequencing, immunoblot blot analyses, and mass spectrometry.
激光捕获显微切割是一种非分子、微创性的方法，用于从异质性组织中获取细胞学和/或表型明确的细胞或细胞群。其优点包括高效、快速且精确地获取细胞。潜在的缺点包括耗时、昂贵，并且受限于需要病理学家来识别特定样本中的不同亚群。总体而言，它用途广泛，能够制备特定细胞亚群的同质分离物，从中可以提取DNA/RNA或蛋白质用于逆转录聚合酶链反应（RT-PCR）、定量聚合酶链反应、下一代测序、免疫印迹分析和质谱分析。
