Decarlo Kristen, Emley Andrew, Dadzie Ophelia E, Mahalingam Meera
Boston University School of Medicine, Boston, MA, USA.
Methods Mol Biol. 2011;755:1-15. doi: 10.1007/978-1-61779-163-5_1.
Laser microdissection is a nonmolecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. It is a versatile technology and allows the preparation of homogenous isolates of specific subpopulations of cells from which RNA/DNA or protein can be extracted for RT-polymerase chain reaction (PCR), quantitative PCR, Western blot analyses, and mass spectrophotometry.
激光显微切割是一种非分子、微创性方法，用于从异质性组织中获取细胞学和/或表型明确的细胞或细胞群。它是一项通用技术，能够制备特定细胞亚群的同质分离物，从中可提取RNA/DNA或蛋白质，用于逆转录聚合酶链反应（RT-PCR）、定量PCR、蛋白质印迹分析和质谱分析。
