Eseyin Olorunfemi A, Sattar Munavvar A, Rathore Hassaan A, Aigbe Flora, Afzal Sheryar, Ahmad Ashfaq, Lazhari Mohammed, Akthar Safia
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Uyo, Akwa Ibom State, Nigeria / Hypertension and Cardiovascular Physiology Research Laboratory, School of Pharmaceutical Sciences, University Sains Malaysia, Penang, Malaysia.
Hypertension and Cardiovascular Physiology Research Laboratory, School of Pharmaceutical Sciences, University Sains Malaysia, Penang, Malaysia.
Pak J Pharm Sci. 2018 Jan;31(1):45-50.
Telfairia occidentalis possesses high antioxidant activity. However, the antioxidant components of the plant have not yet been identified. This study was undertaken to identify the phenolics in the leaf of the plant. Extract and fractions of the leaf of the plant were analysed using the HPLC and GCMS. HPLC analysis revealed the presence of gallic acid (22.19μg/mg), catechin (29.17μg/mg), caffeic acid (9.17μg/mg), ferulic acid (0.94μg/mg), sinapic acid (1.91 μg/mg) and 4-hydroxy benzoic acid (43.86 μg/mg) in the aqueous extract. Phenolics fraction contained gallic acid (0.88 μg/mg), catechin (2.70μg/mg), caffeic acid (7.92μg/mg), ferulic acid (2.72μg/mg), benzoic acid (6.36μg/mg), p-coumaric acid (1.48μg/mg), quercetin (12.00μg/mg). Only caffeic acid (2.50μg/mg), ferulic acid (0.44μg/mg) and quercetin (8.50μg/mg) were detected in the flavonoid fraction. While GCMS analysis showed the presence of methylparaben; ethylparaben; benzoic acid; 4-hydroxy-2-methoxy-3,5,6-trimethyl-, methyl ester; 4-hydroxy-3-methoxy; phenol, 5-methoxy-2-(methoxymethyl)-; phenol, 5-methoxy-2, 3- dimethyl; and phenol, 2-(2-benzothiazolyl)-. This study is the first to reveal the identity of some phenolics components of the leaf of Telfairia occidentalis.
西非油瓜具有很高的抗氧化活性。然而，该植物的抗氧化成分尚未得到鉴定。本研究旨在鉴定该植物叶片中的酚类物质。使用高效液相色谱法（HPLC）和气相色谱 - 质谱联用仪（GCMS）对该植物叶片的提取物和馏分进行了分析。HPLC分析显示，水提取物中存在没食子酸（22.19μg/mg）、儿茶素（29.17μg/mg）、咖啡酸（9.17μg/mg）、阿魏酸（0.94μg/mg）、芥子酸（1.91μg/mg）和4 - 羟基苯甲酸（43.86μg/mg）。酚类馏分含有没食子酸（0.88μg/mg）、儿茶素（2.70μg/mg）、咖啡酸（7.92μg/mg）、阿魏酸（2.72μg/mg）、苯甲酸（6.36μg/mg）、对香豆酸（1.48μg/mg）、槲皮素（12.00μg/mg）。在黄酮类馏分中仅检测到咖啡酸（2.50μg/mg）、阿魏酸（0.44μg/mg）和槲皮素（8.50μg/mg）。而GCMS分析表明存在对羟基苯甲酸甲酯；对羟基苯甲酸乙酯；苯甲酸；4 - 羟基 - 2 - 甲氧基 - 3,5,6 - 三甲基 - 甲酯；4 - 羟基 - 3 - 甲氧基；5 - 甲氧基 - 2 - （甲氧基甲基）苯酚；5 - 甲氧基 - 2,3 - 二甲基苯酚；以及2 - （2 - 苯并噻唑基）苯酚。本研究首次揭示了西非油瓜叶片中一些酚类成分的身份。
