Salman Toyin Mohammed, Iyanda Mayowa Adewale, Alli-Oluwafuyi Abdul-Musawwir, Sulaiman Sheu Oluwadare, Alagbonsi Abdullateef Isiaka
Department of Physiology, College of Health Sciences, University of Ilorin, Ilorin, Nigeria.
Department of Pharmacology and Therapeutics, College of Health Sciences, University of Ilorin, Ilorin, Nigeria.
Metabol Open. 2021 Apr 14;10:100092. doi: 10.1016/j.metop.2021.100092. eCollection 2021 Jun.
, a plant consumed for its nutritional and medicinal values, exhibits hypoglycaemic effect. However, the metabolic fate of the glucose following TO-induced insulin secretion and consequent hypoglycaemia is not clear.
This study determined the effect of ethyl acetate and n-hexane fractions of TO leaf extracts on some biochemical parameters in the glucose metabolic pathway to explain the possible fate of blood glucose following TO-induced hypoglycaemia.
Eighteen male Wistar rats (180-200 g) divided into control, n-hexane TO fraction- and ethyl acetate TO fraction-treated groups (n = 6/group) were used. The control animals received normal saline while the treated groups received TO at 100 mg/kg for seven days. After 24 h following the last dose, the animals were anaesthetised using ketamine; blood samples were collected and livers harvested to determine some biochemical parameters.
Ethyl acetate TO fraction significantly increased plasma insulin, liver glucokinase activity and plasma pyruvate concentration, but significantly decreased plasma glucose and liver glycogen, without significant changes in plasma lactate, glucose-6-phosphate, liver glucose-6-phosphatase and lactate dehydrogenase activities when compared with control. N-hexane TO fraction significantly reduced liver glucose-6-phosphatase activity and glycogen but significantly increased plasma pyruvate, without significant changes in plasma glucose, insulin, glucose-6-phosphate and lactate concentrations; and liver glucokinase and lactate dehydrogenase activities.
The present study showed that insulin-mediated TO-induced hypoglycaemia resulted in the stimulation of glycolysis and pyruvate production via insulin-dependent and insulin-independent mechanisms.
[植物名称]因其营养和药用价值而被食用，具有降血糖作用。然而，[植物名称]诱导胰岛素分泌及随之而来的低血糖后葡萄糖的代谢命运尚不清楚。
本研究测定了[植物名称]叶提取物的乙酸乙酯和正己烷馏分对葡萄糖代谢途径中一些生化参数的影响，以解释[植物名称]诱导低血糖后血糖的可能命运。
选用18只雄性Wistar大鼠（180 - 200 g），分为对照组、正己烷[植物名称]馏分组和乙酸乙酯[植物名称]馏分组（每组n = 6）。对照动物接受生理盐水，处理组接受100 mg/kg的[植物名称]，持续7天。最后一次给药后24小时，用氯胺酮麻醉动物；采集血样并摘取肝脏以测定一些生化参数。
与对照组相比，乙酸乙酯[植物名称]馏分显著增加血浆胰岛素、肝葡萄糖激酶活性和血浆丙酮酸浓度，但显著降低血浆葡萄糖和肝糖原，而血浆乳酸、6 - 磷酸葡萄糖、肝葡萄糖 - 6 - 磷酸酶和乳酸脱氢酶活性无显著变化。正己烷[植物名称]馏分显著降低肝葡萄糖 - 6 - 磷酸酶活性和糖原，但显著增加血浆丙酮酸，血浆葡萄糖、胰岛素、6 - 磷酸葡萄糖和乳酸浓度以及肝葡萄糖激酶和乳酸脱氢酶活性无显著变化。
本研究表明，胰岛素介导的[植物名称]诱导低血糖通过胰岛素依赖和非胰岛素依赖机制刺激糖酵解和丙酮酸生成。
