Chan Yik Sin, Cheah Yit Hong, Chong Poh Zen, Khor Hui Lai, Teh Wen Siang, Khoo Kong Soo, Ong Hean Chooi, Sit Nam Weng
Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Bandar Barat, Kampar, Perak, Malaysia.
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Pak J Pharm Sci. 2018 Jan;31(1):119-127.
This study was conducted to investigate the antifungal potential and cytotoxicity of selected medicinal plants from Malaysia. The extracts from the stem of Cissus quadrangularis and the leaves of Asplenium nidus, Pereskia bleo, Persicaria odorata and Sauropus androgynus were assayed against six fungi using p-iodonitrotetrazolium-based on colorimetric broth microdilution method. All the plant extracts were found to be fungicidal against at least one type of fungus. The strongest fungicidal activity (minimum fungicidal concentration=0.16 mg/mL) were exhibited by the hexane extract of C. quadrangularis, the hexane, chloroform, ethanol and methanol extracts of P. bleo, the hexane and ethyl acetate extracts of P. odorata, and the water extract of A. nidus. In terms of cytotoxicity on the African monkey kidney epithelial (Vero) cells, the chloroform extract of P. odorata produced the lowest 50% cytotoxic concentration (100.3 ± 4.2 μ g/mL). In contrast, none of the water extracts from the studied plants caused significant toxicity on the cells. The water extract of A. nidus warrants further investigation since it showed the strongest fungicidal activity and the highest total activity (179.22 L/g) against Issatchenkia orientalis, and did not cause any toxicity to the Vero cells.
本研究旨在调查马来西亚选定药用植物的抗真菌潜力和细胞毒性。采用基于比色肉汤微量稀释法的对碘硝基四氮唑，对四角叶白粉藤茎提取物以及鸟巢蕨、虎刺、假荜茇和白背叶叶提取物进行了六种真菌的检测。发现所有植物提取物对至少一种真菌具有杀菌作用。四角叶白粉藤的己烷提取物、虎刺的己烷、氯仿、乙醇和甲醇提取物、假荜茇的己烷和乙酸乙酯提取物以及鸟巢蕨的水提取物表现出最强的杀菌活性（最低杀菌浓度 = 0.16 mg/mL）。就对非洲绿猴肾上皮（Vero）细胞的细胞毒性而言，假荜茇的氯仿提取物产生的最低50%细胞毒性浓度为（100.3 ± 4.2 μg/mL）。相比之下，所研究植物的水提取物均未对细胞造成明显毒性。鸟巢蕨的水提取物值得进一步研究，因为它对东方伊萨酵母表现出最强的杀菌活性和最高的总活性（179.22 L/g），并且对Vero细胞没有任何毒性。
