Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia.
Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia.
Int Microbiol. 2016 Sep;19(3):175-182. doi: 10.2436/20.1501.01.275.
Chikungunya virus is a reemerging arbovirus transmitted mainly by Aedes mosquitoes. As there are no specific treatments available, Chikungunya virus infection is a significant public health problem. This study investigated 120 extracts from selected medicinal plants for anti-Chikungunya virus activity. The plant materials were subjected to sequential solvent extraction to obtain six different extracts for each plant. The cytotoxicity and antiviral activity of each extract were examined using African monkey kidney epithelial (Vero) cells. The ethanol, methanol and chloroform extracts of Tradescantia spathacea (Commelinaceae) leaves showed the strongest cytopathic effect inhibition on Vero cells, resulting in cell viabilities of 92.6% ± 1.0% (512 μg/ml), 91.5% ± 1.7% (512 μg/ml) and 88.8% ± 2.4% (80 μg/ml) respectively. However, quantitative RT-PCR analysis revealed that the chloroform extract of Rhapis excelsa (Arecaceae) leaves resulted in the highest percentage of reduction of viral load (98.1%), followed by the ethyl acetate extract of Vernonia amygdalina (Compositae) leaves (95.5%). The corresponding 50% effective concentrations (EC50) and selectivity indices for these two extracts were 29.9 ± 0.9 and 32.4 ± 1.3 μg/ml, and 5.4 and 5.1 respectively. Rhapis excelsa and Vernonia amygdalina could be sources of anti-Chikungunya virus agents. [Int Microbiol 19(3):175-182 (2016)].
基孔肯雅热病毒是一种主要由埃及伊蚊传播的新兴虫媒病毒。由于目前尚无特效治疗方法，基孔肯雅热病毒感染已成为一个严重的公共卫生问题。本研究调查了 120 种选定药用植物提取物的抗基孔肯雅病毒活性。对植物材料进行连续溶剂萃取，得到每种植物的 6 种不同提取物。用非洲绿猴肾上皮（Vero）细胞检测每种提取物的细胞毒性和抗病毒活性。Tradescantia spathacea（鸭跖草科）叶的乙醇、甲醇和氯仿提取物对 Vero 细胞的致病变效应抑制作用最强，细胞活力分别为 92.6%±1.0%（512μg/ml）、91.5%±1.7%（512μg/ml）和 88.8%±2.4%（80μg/ml）。然而，定量 RT-PCR 分析显示，Rhapis excelsa（棕榈科）叶的氯仿提取物导致病毒载量降低的百分比最高（98.1%），其次是 Vernonia amygdalina（菊科）叶的乙酸乙酯提取物（95.5%）。这两种提取物的 50%有效浓度（EC50）和选择性指数分别为 29.9±0.9 和 32.4±1.3μg/ml，5.4 和 5.1。Rhapis excelsa 和 Vernonia amygdalina 可能是抗基孔肯雅病毒药物的来源。[Int Microbiol 19(3):175-182 (2016)]。
