Wang Bing, Ma Ling, Yin Lei, Chen Jing, Zhang Yuanbin, Dong Lin, Zhang Xinhui, Fu Xueyan
School of Pharmacy, Ningxia Medical University, Yinchuan, China.
School of Pharmacy, Ningxia Medical University, Yinchuan, China / Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine, Yinchuan, China / Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, China.
Pak J Pharm Sci. 2018 Jan;31(1):221-229.
Rosemary is an aromatic evergreen shrubby herb that is native to the Mediterranean region. This herb is now widely cultivated in many regions of the world. Rosemary is widely used in traditional Chinese medicines, foods, nutraceuticals and cosmetics. Hydro distilled essential oils, obtained from rosemary in China and the Mediterranean region, were analysed by gas chromatography-mass spectrometry (GC-MS). Thirty-seven compounds accounting for 94.97%-99.72% of the oils were identified. The majority of the compounds in the essential oils exhibited no significant differences (table 1 and fig. 1). The extracts were prepared with three solvents of different polarity (dichloromethane, ethyl acetate and aqueous). The ethyl acetate fractions exhibited the highest phenol content and were found to be significantly more active than the dichloromethane and aqueous fractions (fig. 2). Antioxidant activity (by DPPH radical scavenging, ferric ion reduction (FRAP) and thiobarbituric acid reactive substance (TBARS)) was also assessed. The ethyl acetate extracts of Yunnan had the highest amount of antioxidant capacity from China by DPPH and TBARS, with the lowest IC50 values being 0.0011 mg/ml, and 1.6611 mg/ml, respectively. In conclusion, the antioxidant activities of the essential oils and ethyl acetate extracts from rosemary obtained by three different testing methods revealed higher antioxidant activity from rosemary grown in China than in the Mediterranean region. These results suggested that Chinese rosemary should be widely used in food, traditional medicine, cosmetics and perfume products, as well as other chemical industries.
迷迭香是一种芳香常绿灌木状草本植物，原产于地中海地区。这种草本植物现在在世界许多地区广泛种植。迷迭香广泛应用于中药、食品、营养保健品和化妆品中。对在中国和地中海地区从迷迭香中通过水蒸馏法获得的精油进行了气相色谱 - 质谱联用（GC - MS）分析。鉴定出了占精油94.97% - 99.72%的37种化合物。精油中的大多数化合物没有显著差异（表1和图1）。用三种不同极性的溶剂（二氯甲烷、乙酸乙酯和水）制备提取物。乙酸乙酯馏分的酚含量最高，并且发现其活性明显高于二氯甲烷馏分和水馏分（图2）。还评估了抗氧化活性（通过DPPH自由基清除、铁离子还原（FRAP）和硫代巴比妥酸反应性物质（TBARS））。云南的乙酸乙酯提取物通过DPPH和TBARS测定在中国具有最高的抗氧化能力，最低IC50值分别为0.0011mg/ml和1.6611mg/ml。总之，通过三种不同测试方法获得的迷迭香精油和乙酸乙酯提取物的抗氧化活性表明，中国种植的迷迭香比地中海地区种植的迷迭香具有更高的抗氧化活性。这些结果表明，中国迷迭香应广泛用于食品、传统医药、化妆品和香水产品以及其他化学工业。
