Long Feng, Wang Liangliang, Lupa Boguslaw, Whitman William B
Department of Microbiology, University of Georgia, Athens, GA 30602-2605, USA.
Archaea. 2017 Nov 19;2017:7046026. doi: 10.1155/2017/7046026. eCollection 2017.
Many hydrogenotrophic methanogens use either H or formate as the major electron donor to reduce CO for methane production. The conventional cultivation of these organisms uses H and CO as the substrate with frequent replenishment of gas during growth. H is explosive and requires an expensive gassing system to handle safely. Formate is as an ideal alternative substrate from the standpoints of both economy and safety but leads to large changes in the culture pH during growth. Here, we report that glycylglycine is an inexpensive and nontoxic buffer suitable for growth of and . This cultivation system is suitable for growth on liquid as well as solid medium in serum bottles. Moreover, it allows cultivation of liter scale cultures without expensive fermentation equipment. This formate cultivation system provides an inexpensive and flexible alternative for the growth of formate-utilizing, hydrogenotrophic methanogens.
许多嗜氢产甲烷菌利用氢气或甲酸作为主要电子供体来还原二氧化碳以产生甲烷。这些微生物的传统培养使用氢气和二氧化碳作为底物，在生长过程中需要频繁补充气体。氢气具有爆炸性，需要昂贵的通气系统来安全处理。从经济和安全的角度来看，甲酸是一种理想的替代底物，但在生长过程中会导致培养基pH值发生较大变化。在此，我们报告甘氨酰甘氨酸是一种廉价且无毒的缓冲剂，适用于[具体菌种1]和[具体菌种2]的生长。这种培养系统适用于血清瓶中液体和固体培养基上的生长。此外，它允许在不使用昂贵发酵设备的情况下培养升规模的培养物。这种甲酸培养系统为利用甲酸的嗜氢产甲烷菌的生长提供了一种廉价且灵活的替代方法。
