创伤后应激障碍样综合征大鼠海马体中不同时间点长链非编码RNA表达的差异。
Differences in the expression of long noncoding RNAs at different time points in the PTSD-like syndrome rat hippocampus.
作者信息
Liu Qingzhen, Ji Qing, Liu Jian, Zhang Lidong
机构信息
Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, China.
出版信息
Oncotarget. 2017 Sep 18;8(68):112460-112466. doi: 10.18632/oncotarget.21025. eCollection 2017 Dec 22.
The aim of this study was to characterize the expression profiles at different time points in the PTSD-like syndrome rat hippocampus and perform analyses. PTSD rat models were made as reported by Rau, and we collected the hippocampus at different time points. The lncRNAs at different time points were compared by microarray and listed. We used quantitative real-time PCR to confirm the lncRNA profiling expression data. Bioinformatics analysis was performed on EU056364_P1. Compare with control, a total of 948 lncRNAs and 2514 mRNAs were found (fold-change > 2.0) among the four time points. Additionally, bioinformatics analysis of EU056364_P1 suggested it might be involved in memory development through the target gene Camk2a.This study revealed different lncRNAs expressed at different time points in PTSD and explored the targets of PTSD.
本研究的目的是表征创伤后应激障碍（PTSD）样综合征大鼠海马体在不同时间点的表达谱并进行分析。按照劳（Rau）报道的方法制作PTSD大鼠模型，我们在不同时间点采集海马体。通过微阵列比较不同时间点的长链非编码RNA（lncRNA）并列出。我们使用定量实时聚合酶链反应（qRT-PCR）来确认lncRNA谱表达数据。对EU056364_P1进行生物信息学分析。与对照组相比，在四个时间点共发现948个lncRNA和2514个信使核糖核酸（mRNA）（变化倍数>2.0）。此外，对EU056364_P1的生物信息学分析表明，它可能通过靶基因钙/钙调蛋白依赖蛋白激酶2α（Camk2a）参与记忆发育。本研究揭示了PTSD在不同时间点表达的不同lncRNA，并探索了PTSD的靶点。
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