Li Guizhen, Wang Xiaoqin, Row Kyung Ho
Department of Chemistry and Chemical Engineering, Inha University, Nam-Ku, Incheon, Korea.
Electrophoresis. 2018 Apr;39(8):1111-1118. doi: 10.1002/elps.201700474. Epub 2018 Feb 6.
Novel magnetic molecularly imprinted polymers (MMIPs) with multiple-template based on silica were modified by four types of deep eutectic solvents (DESs) for the rapid simultaneous magnetic solid-phase extraction (MSPE) of tanshinone Ⅰ, tanshinone ⅡA, and cryptotanshinone from Salvia miltiorrhiza bunge; glycitein, genistein, and daidzein from Glycine max (Linn.) Merr; and epicatechin, epigallocatechin gallate, and epicatechin gallate from green tea, respectively. The synthesized materials were characterized by Fourier transform infrared spectroscopy and field emission scanning electron microscopy. Single factor experiments were to explore the relationship between the extraction efficiency and four factors (the sample solution pH, amount of DESs for modification, amount of adsorbent, and extraction time). It was showed that the DES4-MMIPs have better extraction ability than the MMIPs without DESs and the other three DESs-modified MMIPs. The best extraction recoveries with DES4-MMIP were tanshinone Ⅰ (85.57%), tanshinone ⅡA (80.58%), cryptotanshinone (92.12%), glycitein (81.65%), genistein (87.72%), daidzein (92.24%), epicatechin (86.43%), epigallocatechin gallate (80.92%), and epicatechin gallate (93.64%), respectively. The novel multiple-template MMIPs materials modified by DES for the rapid simultaneous MSPE of active compounds were proved to reduce the experimental steps than single-template technique, and increase the extraction efficiency.
基于二氧化硅的新型多模板磁性分子印迹聚合物（MMIPs）用四种类型的低共熔溶剂（DESs）进行改性，用于从丹参中快速同时磁性固相萃取（MSPE）丹参酮Ⅰ、丹参酮ⅡA和隐丹参酮；从大豆中快速同时磁性固相萃取大豆黄素、染料木素和黄豆苷元；以及从绿茶中快速同时磁性固相萃取表儿茶素、表没食子儿茶素没食子酸酯和儿茶素没食子酸酯。通过傅里叶变换红外光谱和场发射扫描电子显微镜对合成材料进行了表征。单因素实验旨在探究萃取效率与四个因素（样品溶液pH值、改性DESs用量、吸附剂用量和萃取时间）之间的关系。结果表明，DES4-MMIPs比未使用DESs的MMIPs以及其他三种DESs改性的MMIPs具有更好的萃取能力。使用DES4-MMIP时，丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮、大豆黄素、染料木素、黄豆苷元、表儿茶素、表没食子儿茶素没食子酸酯和儿茶素没食子酸酯的最佳萃取回收率分别为85.57%、80.58%、92.12%、81.65%、87.72%、92.24%、86.43%、80.92%和93.64%。经证明，用于活性化合物快速同时MSPE的DES改性新型多模板MMIPs材料比单模板技术减少了实验步骤，并提高了萃取效率。
