Qin Shili, Jin Fenglong, Gao Lidi, Su Liqiang, Li Yingjie, Han Shuang, Wang Peng
College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang 161006, People's Republic of China.
Laboratory of Food testing, Qiqihar Food and Drug Administration, Bukui South Street 297, Longsha District, Qiqihar 161006, People's Republic of China.
R Soc Open Sci. 2019 Jun 26;6(6):190119. doi: 10.1098/rsos.190119. eCollection 2019 Jun.
A molecularly imprinted monolith was prepared and evaluated for the special selective separation of sulfamerazine (SMR) by capillary electrochromatography (CEC). The single-step polymerization method was applied through thermally immobilized vinyl groups of itaconic acid and a derivatization capillary column using SMR as the template. The monolith with optimal selectivity and permeability was performed at 45°C for 7 h according to the molar ratios of 1 : 4 : 10 (template/functional monomer/cross-linker). Under the optimized separation conditions of 75% acetonitrile in 20 mM phosphate buffer with pH 5.0, 15 kV applied voltage and 20°C column temperature, the imprinted monolith showed strong recognition ability for SMR and high column performance. Finally, the molecularly imprinted monolith coupled with the CEC method was successfully developed for the quantification of SMR in aquatic products, which was properly validated by a good linear relationship, recoveries and limit of detection. The coupling technique of the molecularly imprinted technology and CEC achieved pre-treatment enrichment and separation analysis in only one miniaturized chromatographic column.
制备了一种分子印迹整体柱，并通过毛细管电色谱（CEC）对磺胺甲基嘧啶（SMR）进行了特殊选择性分离评估。采用一步聚合法，以衣康酸的热固定乙烯基和以SMR为模板的衍生化毛细管柱进行聚合。根据1:4:10（模板/功能单体/交联剂）的摩尔比，在45°C下进行7小时，制备出具有最佳选择性和渗透性的整体柱。在优化的分离条件下，即在pH 5.0的20 mM磷酸盐缓冲液中含75%乙腈、施加电压15 kV和柱温20°C时，印迹整体柱对SMR表现出很强的识别能力和高柱效。最后，成功开发了分子印迹整体柱与CEC方法联用技术用于水产品中SMR的定量分析，通过良好的线性关系、回收率和检测限进行了适当验证。分子印迹技术与CEC的联用技术仅在一根微型色谱柱中就实现了预处理富集和分离分析。
