Patel Sanjay K S, Gupta Rahul K, Kumar Virendra, Kondaveeti Sanath, Kumar Anurag, Das Devashish, Kalia Vipin Chandra, Lee Jung-Kul
Department of Chemical Engineering, Konkuk University, Seoul, 05029 Republic of Korea.
Indian J Microbiol. 2020 Sep;60(3):318-324. doi: 10.1007/s12088-020-00883-6. Epub 2020 May 22.
Methanol production by co-culture of methanotrophs and was examined from methane, a greenhouse gas. Co-culture exhibited higher methanol yield of 4.72 mM at optimum ratio of and (3:2) compared to individual cultures. The immobilized co-culture within polyvinyl alcohol (PVA) showed relative efficiency of 90.1% for methanol production at polymer concentration of 10% (v/v). The immobilized co-culture cells within PVA resulted in higher bioprocess stability over free cells at different pH, and temperatures. Free and encapsulated co-cultures showed maximum methanol production of 4.81 and 5.37 mM under optimum conditions, respectively. After five cycles of reusage under batch conditions, free and encapsulated co-cultures retained methanol production efficiency of 23.8 and 61.9%, respectively. The present investigation successfully revealed the useful influence of co-culture on the methanol production over pure culture. Further, encapsulation within the polymeric matrix proved to be a better approach for the enhanced stability of the bioprocess.
利用甲烷氧化菌共培养从温室气体甲烷中生产甲醇进行了研究。与单独培养相比，共培养在甲烷氧化菌与[具体菌种]的最佳比例（3:2）下表现出更高的甲醇产量，为4.72 mM。在聚乙烯醇（PVA）中固定化的共培养物在聚合物浓度为10%（v/v）时，甲醇生产的相对效率为90.1%。在不同的pH值和温度下，PVA中固定化的共培养细胞比游离细胞具有更高的生物过程稳定性。游离和包封的共培养物在最佳条件下分别显示出最大甲醇产量为4.81 mM和5.37 mM。在分批条件下重复使用五个循环后，游离和包封的共培养物分别保留了23.8%和61.9%的甲醇生产效率。本研究成功揭示了共培养对甲醇生产相对于纯培养的有益影响。此外，在聚合物基质中包封被证明是提高生物过程稳定性的更好方法。
