Tozzi-Ciancarelli M G, Amicosante G, Menichelli A, Di Giulio S, Del Principe D
Biol Neonate. 1985;48(6):336-40. doi: 10.1159/000242190.
Several reports have appeared showing the possibility of bilirubin-sensitized photodamage. We have extended these observations to platelets. In the presence of 300 microM bilirubin the in vitro irradiation of isolated platelets or platelet-rich plasmas with visible light induced significant lysis as determined by the release of lactate dehydrogenase (LDH). The extent of LDH release was a function of irradiation time, being about 20% after 2 h of irradiation. A loss membrane-bound ATPase activity was also observed at earlier times, indicating that membrane damage was preliminary to the lytic effect. The release of beta-thromboglobulin, induced by close cell-to-cell contact, was lower in bilirubin- and light-treated platelets with respect to controls. Our results suggest that bilirubin may act as a photodynamic agent producing some damage on human blood platelets.
已有多篇报道表明胆红素致敏光损伤的可能性。我们将这些观察结果扩展到了血小板。在存在300微摩尔胆红素的情况下，用可见光对分离的血小板或富含血小板的血浆进行体外照射，通过乳酸脱氢酶（LDH）的释放测定，可诱导显著的溶解。LDH释放程度是照射时间的函数，照射2小时后约为20%。在更早的时间也观察到膜结合ATP酶活性的丧失，表明膜损伤是溶解作用的前奏。在胆红素和光处理的血小板中，由紧密细胞间接触诱导的β-血小板球蛋白释放相对于对照组较低。我们的结果表明，胆红素可能作为一种光动力剂对人血小板产生一些损伤。
