Department of Ecological Plant Protection, University of Kassel, Witzenhausen, Germany.
Department of Organic Plant Breeding and Agrobiodiversity, University of Kassel, Witzenhausen, Germany.
Sci Rep. 2018 Jan 19;8(1):1252. doi: 10.1038/s41598-018-19779-z.
The Fusarium solani species complex (FSSC) has been studied intensively but its association with legumes, particularly under European agro-climatic conditions, is still poorly understood. In the present study, we investigated phylogenetic relationships and aggressiveness of 79 isolates of the FSSC collected from pea, subterranean clover, white clover and winter vetch grown under diverse agro-climatic and soil conditions within Temperate and Mediterranean Europe. The isolates were characterized by sequencing tef1 and rpb2 loci and by greenhouse aggressiveness assays. The majority of the isolates belonged to two lineages: the F. pisi comb. nov. lineage (formerly F. solani f. sp. pisi) mainly accommodating German and Swiss isolates, and the Fusisporium (Fusarium) solani lineage accommodating mainly Italian isolates. Based on the results of aggressiveness tests on pea, most of the isolates were classified as weakly to moderately aggressive. In addition, using one model strain, 62 accessions of 10 legume genera were evaluated for their potential to host F. pisi, the species known mainly as a pathogen of pea. A total of 58 accessions were colonized, with 25 of these being asymptomatic hosts. These results suggest a broad host range for F. pisi and challenge the forma specialis naming system in Fusarium.
镰刀菌属 Solani 种复合体（FSSC）已得到深入研究，但它与豆科植物的关系，特别是在欧洲农业气候条件下，仍了解甚少。本研究调查了来自豌豆、地下三叶草、白三叶草和冬野豌豆的 79 个 FSSC 分离株的系统发育关系和侵袭性，这些分离株是在温带和地中海欧洲不同农业气候和土壤条件下种植的。通过测序 tef1 和 rpb2 基因座和温室侵袭性测定来表征分离株。大多数分离株属于两个谱系：新组合的豌豆禾谷镰刀菌（Fusarium pisi comb. nov.）谱系（以前称为禾谷镰刀菌 f. sp. pisi），主要容纳德国和瑞士的分离株，和 Fusisporium（Fusarium）禾谷镰刀菌谱系，主要容纳意大利的分离株。基于对豌豆侵袭性测试的结果，大多数分离株被分类为弱至中度侵袭性。此外，使用一个模式菌株，评估了 10 个豆科属的 62 个种系，以确定它们作为豌豆病原菌的豌豆禾谷镰刀菌的宿主潜力。共有 58 个种系被定殖，其中 25 个为无症状宿主。这些结果表明豌豆禾谷镰刀菌的宿主范围广泛，并对 Fusarium 中的特殊形式命名系统提出了挑战。
