Zhu Mei-Fei, Xing Xi, Lei Shu, Wu Jian-Nong, Wang Ling-Cong, Huang Li-Quan, Jiang Rong-Lin
Department of Critical Care Medicine, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310006, China.
Evid Based Complement Alternat Med. 2015;2015:639412. doi: 10.1155/2015/639412. Epub 2015 Aug 4.
Sepsis results in high morbidity and mortality. Immunomodulation strategies could be an adjunctive therapy to treat sepsis. Acupuncture has also been used widely for many years in China to treat sepsis. However, the underlying mechanisms are not well-defined. We demonstrated here that EA preconditioning at ST36 obviously ameliorated CLP-induced intestinal injury and high permeability and reduced the mortality of CLP-induced sepsis rats. Moreover, electroacupuncture (EA) pretreatment exerted protective effects on intestinal mucosal immune barrier by increasing the concentration of sIgA and the percentage of CD3+, γ/δ, and CD4+ T cells and the ratio of CD4+/CD8+ T cells. Although EA at ST36 treatments immediately after closing the abdomen in the CLP procedure with low-frequency or high-frequency could not reduce the mortality of CLP-induced sepsis in rats, these EA treatments could also significantly improve intestinal injury index in rats with sepsis and obviously protected intestinal mucosal immune barrier. In conclusion, our findings demonstrated that EA at ST36 could improve intestinal mucosal immune barrier in sepsis induced by CLP, while the precise mechanism underlying the effects needs to be further elucidated.
脓毒症导致高发病率和死亡率。免疫调节策略可能是治疗脓毒症的辅助疗法。针刺在中国也已广泛应用多年来治疗脓毒症。然而，其潜在机制尚未明确。我们在此证明，电针预处理足三里穴明显改善了盲肠结扎穿孔（CLP）诱导的肠道损伤和高通透性，并降低了CLP诱导的脓毒症大鼠的死亡率。此外，电针预处理通过增加分泌型免疫球蛋白A（sIgA）的浓度、CD3⁺、γ/δ和CD4⁺ T细胞的百分比以及CD4⁺/CD8⁺ T细胞的比例，对肠道黏膜免疫屏障发挥保护作用。尽管在CLP手术关腹后立即采用低频或高频电针刺激足三里穴不能降低CLP诱导的脓毒症大鼠的死亡率，但这些电针治疗也可显著改善脓毒症大鼠的肠道损伤指数，并明显保护肠道黏膜免疫屏障。总之，我们的研究结果表明，电针刺激足三里穴可改善CLP诱导的脓毒症中的肠道黏膜免疫屏障，而其作用的精确机制有待进一步阐明。
