利用“tsBN2”突变，可轻松分析处于G2期停滞的细胞的染色体。

Chromosomes of G2 arrested cells are easily analyzed by use of the 'tsBN2' mutation.

作者信息

Ishida R, Takahashi T, Nishimoto T

出版信息

Cell Struct Funct. 1985 Dec;10(4):417-20. doi: 10.1247/csf.10.417.

DOI:10.1247/csf.10.417

Abstract

The tsBN2 cell line, a temperature sensitive (ts) mutant of the BHK21/13 cell line (Nishimoto, T. et al. Somatic Cell Genet. 4, 323-340, 1978) has a ts defect in its regulatory mechanism for the initiation of chromosome condensation, the so-called, premature chromosome condensation (PCC) being induced at a nonpermissive temperature (Nishimoto, T. et al. Cell 15, 475-483, 1978, Nishimoto, T. et al. J. Cell Physiol. 109, 299-308, 1981). Using the 'tsBN2' mutation, we analyzed chromosomes of tsBN2 cells arrested in the G2 phase with neocarzinostatin (NCS) and found considerable aberrations, such as gaps, breaks and double minutes-like fragmentation, in addition to a typical G2-chromosome (a long filamental chromosome made up of two chromatids). Our results provide evidences that the tsBN2 cell line can be used for examinations of the chromosomes of cells arrested in the G2 phase.

摘要

tsBN2细胞系是BHK21/13细胞系的温度敏感（ts）突变体（Nishimoto, T.等人，《体细胞遗传学》4, 323 - 340, 1978），其在染色体凝聚起始的调控机制中存在ts缺陷，即在非允许温度下会诱导所谓的早熟染色体凝聚（PCC）（Nishimoto, T.等人，《细胞》15, 475 - 483, 1978；Nishimoto, T.等人，《细胞生理学杂志》109, 299 - 308, 1981）。利用“tsBN2”突变，我们分析了用新制癌菌素（NCS）阻滞在G2期的tsBN2细胞的染色体，发现除了典型的G2染色体（由两条染色单体组成的长丝状染色体）外，还有相当多的畸变，如缺口、断裂和双微体样片段化。我们的结果提供了证据表明tsBN2细胞系可用于检测阻滞在G2期的细胞的染色体。