Nishimoto T, Ajiro K, Hirata M, Yamashita K, Sekiguchi M
Exp Cell Res. 1985 Feb;156(2):351-8. doi: 10.1016/0014-4827(85)90542-7.
The induction of premature chromosome condensation (PCC) in tsBN2 cells, a temperature-sensitive (ts) mutant of BHK21/13 which shows PCC at the non-permissive temperature, was almost completely inhibited by 40 microM W-7, an antagonist of calmodulin. The mitotic phosphorylation of histone H1 and H3 was also inhibited by W-7. W-5, a chlorine-deficient analogue of W-7 and which interacts weakly with calmodulin, did not inhibit the induction of PCC, even at a dose of 80 microM. The content of calmodulin in tsBN2 cells was increased by a temperature shift to 40.5 degrees C. All these results suggested that calmodulin is required for the chromosome condensation.
tsBN2细胞是BHK21/13的温度敏感（ts）突变体，在非允许温度下会出现早熟染色体凝聚（PCC），40微摩尔的钙调蛋白拮抗剂W-7几乎完全抑制了tsBN2细胞中PCC的诱导。组蛋白H1和H3的有丝分裂磷酸化也受到W-7的抑制。W-5是W-7的缺氯类似物，与钙调蛋白的相互作用较弱，即使剂量为80微摩尔也不抑制PCC的诱导。将tsBN2细胞的温度转移到40.5摄氏度会使其钙调蛋白含量增加。所有这些结果表明，染色体凝聚需要钙调蛋白。
