Yamashita K, Davis F M, Rao P N, Sekiguchi M, Nishimoto T
Cell Struct Funct. 1985 Sep;10(3):259-70. doi: 10.1247/csf.10.259.
In tsBN2 cells, a temperature-sensitive (ts) mutant of the BHK21 cell line, with a ts-defect in its regulatory system for chromosome condensation, antigens that react with mitotic specific mouse monoclonal antibody MPM-2 were produced when premature chromosome condensation (PCC) was induced by a temperature shift. The polypeptides of antigens recognized by MPM-2 in tsBN2 cells with PCC were identical to those of antigens in mitotic cells. These antigens appeared concomitantly with chromosome condensation, which suggests that these mitotic-specific antigens may be related to chromosome condensation. As the production of mitotic-specific antigens was inhibited by W-7, a specific and potent antagonist of calmodulin, calmodulin may function in the mitotic phosphorylation of nonhistone protein.
在tsBN2细胞（BHK21细胞系的一种温度敏感型（ts）突变体，其染色体凝聚调节系统存在ts缺陷）中，当通过温度转换诱导早熟染色体凝聚（PCC）时，会产生与有丝分裂特异性小鼠单克隆抗体MPM - 2发生反应的抗原。在发生PCC的tsBN2细胞中，MPM - 2识别的抗原多肽与有丝分裂细胞中的抗原多肽相同。这些抗原与染色体凝聚同时出现，这表明这些有丝分裂特异性抗原可能与染色体凝聚有关。由于有丝分裂特异性抗原的产生受到钙调蛋白的特异性强效拮抗剂W - 7的抑制，钙调蛋白可能在非组蛋白的有丝分裂磷酸化中发挥作用。
