Lochmanová A, Chmelař D, Beran V, Hájek M
Epidemiol Mikrobiol Imunol. 2017 Fall;66(4):182-188.
Flow cytometry is a method that allows simultaneous measurement and analysis of physical and chemical characteristics of cells or other biological particles during their passage through the laser beam. Although this method is mainly used in the study of cell differentiation and functional analysis of eukaryotic cells, the basic principles of flow cytometry can be applied to microorganisms. Methods based on the analysis of a single cell, such as flow cytometry, in combination with measurement of cell viability using special fluorescent probes allow a deeper insight into the diversity of populations and functioning of microbial communities and also facilitate understanding the phy-siological diversity of seemingly similar acting populations. When using specific fluorescent dyes for the selective labeling of selected species of microorganisms, the method is potentially very specific. The aim of this paper is a brief overview of applications of flow cytometry, which can be used in microbiology.
流式细胞术是一种能够在细胞或其他生物颗粒通过激光束时，同时测量和分析其物理和化学特性的方法。尽管该方法主要用于真核细胞的细胞分化研究和功能分析，但流式细胞术的基本原理也可应用于微生物。基于单细胞分析的方法，如流式细胞术，与使用特殊荧光探针测量细胞活力相结合，能够更深入地了解微生物群落的种群多样性和功能，也有助于理解看似作用相似的种群的生理多样性。当使用特定荧光染料对选定的微生物物种进行选择性标记时，该方法可能具有很高的特异性。本文旨在简要概述可用于微生物学的流式细胞术的应用。
