Fouchet P, Jayat C, Héchard Y, Ratinaud M H, Frelat G
Laboratoire de Cytométrie (LDC), Direction des Sciences du Vivant, CEA, Fontenay-aux-Roses, France.
Biol Cell. 1993;78(1-2):95-109. doi: 10.1016/0248-4900(93)90120-4.
This review focuses on the recent applications of flow cytometry (FCM) in microbiological research (1987-mid 1992). It tries to give a scope of the important breakthroughs which occurred in this field during this period. The technical difficulties of microorganism analysis by flow cytometry is briefly appraised. The significance and the limits of the different microbial cell parameters attainable by flow analyses are systematically evaluated: light scatter for cell size and structure, fluorescence measurements for quantification of cellular components, microbial antigen detection and cell physiological activity estimation. Emphasis is given on the new technological advances which appeared in the last two years. The second part of the review is devoted to the analysis of the usefulness of flow cytometric approach in the different fields of microbiology: fundamental studies in microbial physiology, differentiation, microbial ecology and aquatic sciences, medical microbiology, parasitology, microbial pharmacology and biotechnology.
本综述聚焦于流式细胞术（FCM）在微生物学研究中的近期应用（1987年至1992年年中）。它试图概述这一时期该领域发生的重要突破。简要评估了通过流式细胞术分析微生物的技术难点。系统评估了通过流式分析可获得的不同微生物细胞参数的意义和局限性：用于细胞大小和结构的光散射、用于细胞成分定量的荧光测量、微生物抗原检测和细胞生理活性估计。重点介绍了过去两年出现的新技术进展。综述的第二部分致力于分析流式细胞术方法在微生物学不同领域的实用性：微生物生理学、分化、微生物生态学和水生科学、医学微生物学、寄生虫学、微生物药理学和生物技术的基础研究。
