College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003, Qingdao, PR China.
College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003, Qingdao, PR China.
Carbohydr Polym. 2018 Mar 15;184:154-163. doi: 10.1016/j.carbpol.2017.12.033. Epub 2017 Dec 18.
In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying. Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan. The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge. The composite sponge with good hydrophilic could absorb the moisture in the blood to increase blood concentration and viscosity, and become a semi-swelling viscous colloid to clog the capillaries. Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts. It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction). Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization. The obtained data encourage the use of this composite sponge for wound dressings.
在这项工作中，通过真空冷冻干燥，将羟丁基壳聚糖与壳聚糖物理混合，形成一种多孔海绵状材料，从而制备出一种复合海绵。通过将壳聚糖掺入羟丁基壳聚糖中，开发出了具有亲水性和大孔的复合羟丁基壳聚糖海绵。与壳聚糖海绵和羟丁基壳聚糖海绵相比，复合海绵具有更高的孔隙率（约 85%）、更大的吸水率（约 25 倍）、更好的柔软性和更低的血栓形成指数（BCI）。具有良好亲水性的复合海绵可以吸收血液中的水分，增加血液浓度和粘度，形成半肿胀的粘性胶体，堵塞毛细血管。与 L929 细胞和 HUVEC 细胞的细胞相容性测试表明，复合海绵无细胞毒性，并能促进成纤维细胞的生长。它弥补了羟丁基壳聚糖抗菌效果不佳的缺点，实现了更高水平的抗菌效果（减少>99.99%）。最终，在 Sprague-Dawley 大鼠中的体内评价表明，上皮细胞附着在复合海绵上并渗透到内部，此外，还证明复合海绵（HC-1）具有更好的促进伤口愈合的能力，有助于更快地形成皮肤腺体和上皮化。所得数据鼓励将这种复合海绵用于伤口敷料。
