The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel special Environment, Naval Medical Research Institute, Shanghai 200433, China; Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China.
The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel special Environment, Naval Medical Research Institute, Shanghai 200433, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Int J Biol Macromol. 2018 Feb;107(Pt A):93-104. doi: 10.1016/j.ijbiomac.2017.08.142. Epub 2017 Aug 30.
The present study aimed to prepare a composite dressing composed of collagen, chitosan, and alginate, which may promote wound healing and prevent from seawater immersion. Chitosan-collagen-alginate (CCA) cushion was prepared by paintcoat and freeze-drying, and it was attached to a polyurethane to compose CCA composite dressing. The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated. The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model. Preliminary biosecurity was tested by cytotoxicity and hemocompatibility. The results revealed that CCA cushion had good water absorption and mechanical properties. A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones. On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively. More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly. CCA composite dressing showed no significant cytotoxicity, and favorable hemocompatibility. These results suggested that CCA composite dressing could prevent against seawater immersion and promote wound healing while having a good biosecurity.
本研究旨在制备一种由胶原蛋白、壳聚糖和海藻酸钠组成的复合敷料，以促进伤口愈合并防止海水浸泡。通过涂覆和冷冻干燥制备壳聚糖-胶原蛋白-海藻酸钠（CCA）垫，并将其附着在聚氨酯上以构成 CCA 复合敷料。评估了 CCA 垫的溶胀、孔隙率、降解和机械性能。通过大鼠伤口模型测试了 CCA 复合敷料对伤口愈合和海水预防的影响。通过细胞毒性和血液相容性测试了初步的生物安全性。结果表明，CCA 垫具有良好的吸水性和机械性能。在 CCA 复合敷料处理的大鼠中观察到更高的伤口愈合率，明显高于纱布或壳聚糖处理的大鼠。在第 5 天，CCA 复合敷料、纱布和壳聚糖的愈合率分别为 48.49%±1.07%、28.02%±6.4%和 38.97%±8.53%。在 CCA 复合敷料处理的大鼠的组织学图像中观察到更多的成纤维细胞和完整的再上皮化，并且 EGF、bFGF、TGF-β和 CD31 的表达显著增加。CCA 复合敷料无明显细胞毒性，血液相容性良好。这些结果表明，CCA 复合敷料可以防止海水浸泡，促进伤口愈合，同时具有良好的生物安全性。
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