Huang Yiran, Zheng Wenlu, Li Xiaoyu
Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 2199 Lishui Road West, Shenzhen, Guangdong 518055, China.
Anal Biochem. 2018 Mar 15;545:84-90. doi: 10.1016/j.ab.2018.01.010. Epub 2018 Jan 17.
DNA-based probes are powerful analytical tools for protein detection and analysis. Target-induced DNA assembly is a widely used strategy to transduce target-ligand binding to detectable signals. However, most of the existing methods based on DNA assembly require two or more binding sites on the target protein. Here we report a novel detection method suitable for protein targets with just a single binding site. This method is based on target-induced probe assembly, DNA-templated photo-crosslinking, and DNA-mediated toehold strand displacement to form a tri-probe complex that is specific for target protein.
基于DNA的探针是用于蛋白质检测和分析的强大分析工具。靶标诱导的DNA组装是一种广泛使用的将靶标-配体结合转化为可检测信号的策略。然而，大多数现有的基于DNA组装的方法需要靶蛋白上有两个或更多的结合位点。在此，我们报道了一种适用于只有单个结合位点的蛋白质靶标的新型检测方法。该方法基于靶标诱导的探针组装、DNA模板光交联和DNA介导的链置换，以形成对靶蛋白具有特异性的三探针复合物。
