Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Neurol Sci. 2018 Apr;39(4):647-654. doi: 10.1007/s10072-018-3250-4. Epub 2018 Jan 20.
CD4CD25 regulatory T (Treg) cells and Th17 cells play important roles in peripheral immunity. Immune responses are main elements in the pathogenesis of ischemic stroke (IS). The contribution of Th17 cells in IS patients has not been proved, and whether the balance of Treg/Th17 cells is changed in IS patients remains unidentified. In the present study, we studied Th17 and Treg cell frequency, cytokine secretion, expression of transcription factors, and microRNAs related to Th17 and Treg cells differentiation, which is compared between IS patients and control group. Thirty patients with IS and 30 individuals as control group were enrolled in this study. The frequency of Th17 and Treg lymphocytes, the expression of transcription factors and microRNAs related to these cells, and the serum levels of associated cytokines were assessed by flow cytometry, real-time PCR, and ELISA, respectively. A significant reduction in proportion of peripheral Treg cell frequency and the levels of TGF-β and FOXP3 expression were observed in patients with IS compared with controls, while the proportions of Th17 were increased dramatically, and these effects were along with increases in the levels of IL-17A and RORγt expression in IS patients. The levels of mir-326 and mir-106b-25 expression were increased in patients with IS. These studies suggest that the increase in proportion of Th17 cells and decrease in Treg cells might contribute to the pathogenesis of IS. Manipulating the balance between Tregs and Th17 cells might be helpful for the treatment of IS.
CD4CD25 调节性 T（Treg）细胞和 Th17 细胞在外周免疫中发挥重要作用。免疫反应是缺血性中风（IS）发病机制的主要因素。Th17 细胞在 IS 患者中的作用尚未得到证实，IS 患者中 Treg/Th17 细胞的平衡是否发生变化仍不清楚。在本研究中，我们研究了 Th17 和 Treg 细胞频率、细胞因子分泌、与 Th17 和 Treg 细胞分化相关的转录因子和 microRNAs 的表达，比较了 IS 患者和对照组之间的差异。本研究纳入了 30 例 IS 患者和 30 名对照组个体。通过流式细胞术、实时 PCR 和 ELISA 分别评估 Th17 和 Treg 淋巴细胞的频率、与这些细胞相关的转录因子和 microRNAs 的表达以及相关细胞因子的血清水平。与对照组相比，IS 患者外周 Treg 细胞频率和 TGF-β 及 FOXP3 表达水平显著降低，而 Th17 比例显著增加，这些效应伴随着 IS 患者中 IL-17A 和 RORγt 表达水平的升高。IS 患者中 mir-326 和 mir-106b-25 的表达水平升高。这些研究表明，Th17 细胞比例的增加和 Treg 细胞的减少可能有助于 IS 的发病机制。调节 Tregs 和 Th17 细胞之间的平衡可能有助于 IS 的治疗。
