Yan Haoyue, Hua Yini, Ni Jinxia, Wu Xiaona, Xu Jingni, Zhang Ziniu, Dong Juwei, Xiong Zhihao, Yang Lei, Yuan Hongwei
Department of Acupuncture and Moxibustion, Dongzhimen Hospital of Beijing University of Chinese Medicine, China.
Department of Rehabilitation, Beijing Fengtai Hospital, China.
IBRO Neurosci Rep. 2025 Feb 17;18:443-452. doi: 10.1016/j.ibneur.2025.02.007. eCollection 2025 Jun.
Acute ischemic stroke(AIS) is a major life-threatening disease.Some studies have found that AIS may be related to gut flora and immune responses. Acupuncture is used widely in the treatment of AIS. However its relevant mechanism is unclear enough. Therefore, in this study, we wanted to confirm that acupuncture was treating AIS through gut flora and immune response.
We randomly divided 18 rats into equal three groups, including Sham, Middle Cerebral Artery Occlusion (MCAO) and Acupuncture.Rats in the Acupuncture group for a continuous period of three days after surgery. Neurological deficits were assessed using Longa's method, and detection of intestinal flora by 16s rRNA gene sequencing, determination of SCFAs by gas chromatography-mass spectrometry, detection of HDAC and inflammatory cytokines by elisa assay, detection of Th17 and Treg cells by flow cytometry and, observation of pathological and morphological changes in brain and colon tissues by HE staining.
Acupuncture improved the degree of impaired neurological function in MCAO rats and regulated the type and abundance of intestinal bacteria, increased SCFAs of MCAO rats, decreased HDAC1 and HDAC2, modulated the Th17/Treg imbalance, reduced the level of inflammatory factors in the peripheral blood and altered the pathology of the intestine and brain.
Acupuncture repaired neurologic deficits after AIS and may be associated with an immune-inflammatory response mediated by gut microbiota.
急性缺血性卒中（AIS）是一种严重威胁生命的疾病。一些研究发现，AIS可能与肠道菌群和免疫反应有关。针灸在AIS治疗中被广泛应用。然而，其相关机制尚不清楚。因此，在本研究中，我们想证实针灸是通过肠道菌群和免疫反应来治疗AIS的。
我们将18只大鼠随机分为三组，每组数量相等，包括假手术组、大脑中动脉闭塞（MCAO）组和针灸组。针灸组大鼠在手术后连续三天接受针灸治疗。采用Longa法评估神经功能缺损，通过16s rRNA基因测序检测肠道菌群，用气相色谱-质谱法测定短链脂肪酸（SCFAs），用酶联免疫吸附测定法检测组蛋白去乙酰化酶（HDAC）和炎性细胞因子，用流式细胞术检测辅助性T细胞17（Th17）和调节性T细胞（Treg），并用苏木精-伊红（HE）染色观察脑和结肠组织的病理和形态学变化。
针灸改善了MCAO大鼠神经功能受损程度，调节了肠道细菌的类型和丰度，增加了MCAO大鼠的SCFAs，降低了HDAC1和HDAC2，调节了Th17/Treg失衡，降低了外周血中炎性因子水平，并改变了肠道和脑的病理状态。
针灸可修复AIS后的神经功能缺损，可能与肠道微生物群介导的免疫炎症反应有关。
