Jin Z W, Kumar A, Cleveland R P, Murray D L, Kaufman D B
Clin Immunol Immunopathol. 1986 Mar;38(3):350-6. doi: 10.1016/0090-1229(86)90245-x.
Immunomodulatory effects of cimetidine, an H2 histamine receptor antagonist, have been reported in humans and animals. To define these effects more clearly, the action of cimetidine on suppressor cell function was studied utilizing a murine model of contact hypersensitivity. Intravenous inoculation of BALB/c mice with DNP-coupled syngeneic spleen cells induced the production of DNP-specific suppressor cells which could easily be demonstrated by a reduction in ear swelling after contact sensitization with 1-fluoro-2,4-dinitrobenzene (DNFB) following transfer of spleen and lymph node cells to naive syngeneic recipients. Cimetidine treatment of animals in which suppressor cells were induced resulted in an inability of these mice to transfer cellular suppression as measured by development of a normal immunologic response in the recipient mice. The effect of cimetidine was both dose and time related. While all groups receiving cimetidine showed some loss of suppressor cell function, the maximum effect (up to 100% inhibition) was seen when 50 mg/kg of cimetidine was administered intraperitoneally 2 days before or on the day of suppressor cell induction. Some restoration also occurred when cimetidine was given after the day of induction. It has been shown that suppressor cells possess histamine receptors which may be involved in suppressor cell activation. The results indicate that cimetidine may inhibit the functioning of these receptors.
H2组胺受体拮抗剂西咪替丁的免疫调节作用已在人和动物中有所报道。为了更清楚地界定这些作用，利用接触性超敏反应的小鼠模型研究了西咪替丁对抑制细胞功能的作用。用二硝基苯（DNP）偶联的同基因脾细胞静脉接种BALB/c小鼠，可诱导产生DNP特异性抑制细胞，在用1-氟-2,4-二硝基苯（DNFB）进行接触致敏后，将脾细胞和淋巴结细胞转移至同基因未致敏受体小鼠，通过耳肿胀减轻可轻易证明这种抑制细胞的存在。对诱导产生抑制细胞的动物进行西咪替丁治疗后，这些小鼠无法转移细胞抑制作用，这可通过受体小鼠中正常免疫反应的发展来衡量。西咪替丁的作用与剂量和时间相关。虽然所有接受西咪替丁治疗的组均显示出一定程度的抑制细胞功能丧失，但在抑制细胞诱导前2天或诱导当天腹腔注射50mg/kg西咪替丁时，观察到最大效应（高达100%抑制）。在诱导当天之后给予西咪替丁时，也会出现一定程度的恢复。已有研究表明，抑制细胞具有组胺受体，这些受体可能参与抑制细胞的激活。结果表明，西咪替丁可能会抑制这些受体的功能。
