Department of Biotechnology, College of Engineering and Technology, Biju Pattnaik University of Technology, Bhubaneswar 751003, India.
Department of Microbiology, Vidyasagar University, Midnapore 721102, India.
Bioresour Technol. 2018 Apr;253:262-272. doi: 10.1016/j.biortech.2018.01.023. Epub 2018 Jan 6.
A cellulose degrading fungus Aspergillus fumigatus (CWSF-7) isolated from decomposed lignocellulosic waste containing soil was found to produce high titer of cellulases. The optimum activity of CMCase and FPase were 1.9 U/mL and 0.9 U/mL respectively while the highest protein concentration was found to be 1.2 mg/mL. Saccharification of two Pennisetum grass varieties [dennanath (DG) and hybrid napier grass (HNG)] were optimized using partially purified CMCase and FPase in equal concentration, i.e. a ratios of 1:1 and further with addition of commercial xylanase using response surface methodology (RSM). The production of total reducing sugar (TRS) using isolated cellulase were 396.6 and 355.8 (mg/g), whereas further addition of xylanase had higher TRS titers of 478.7 and 483.3 (mg/g) for DG and HNG respectively as evident from HPLC analysis. Further, characterization of the enzyme saccharified DG and HNG by SEM and ATR-FTIR revealed efficient hydrolysis of cellulose and partially hydrolysis of hemicellulose.
从含有土壤的分解木质纤维素废物中分离到的纤维素降解真菌烟曲霉（CWSF-7）被发现能产生高浓度的纤维素酶。CMC 酶和 FP 酶的最适活性分别为 1.9 U/mL 和 0.9 U/mL，而最高的蛋白质浓度被发现为 1.2 mg/mL。使用部分纯化的 CMC 酶和 FP 酶以相等的浓度（即 1:1 的比例）优化了两种狼尾草品种（dennanath（DG）和杂交象草（HNG）的糖化作用，并进一步使用商业木聚糖酶添加响应面法（RSM）。使用分离的纤维素酶产生的总还原糖（TRS）分别为 396.6 和 355.8（mg/g），而进一步添加木聚糖酶后，DG 和 HNG 的 TRS 浓度分别为 478.7 和 483.3（mg/g），这从 HPLC 分析中可以明显看出。此外，通过 SEM 和 ATR-FTIR 对酶糖化的 DG 和 HNG 的特性进行分析，揭示了纤维素的有效水解和半纤维素的部分水解。
