Zou Dehui, Chen Yupei, Liu Tong, Lu Zongxiao, Yan Jun, Chen Dongli, Xu Yue, Zhang Jiayi, Bai Yuzhuo, Zhang Li, Huo Zejun
College of Acupuncture-Moxibustion and Tuina, Beijing University of CM, Beijing 100029, China.
Department of Acupuncture-Moxibustion and Rehabilitation, the Second TCM Hospital of Guangdong Province, Guangzhou 510095, China.
Zhongguo Zhen Jiu. 2017 Sep 12;37(9):971-6. doi: 10.13703/j.0255-2930.2017.09.017.
To observe effects of electroacupuncture (EA) at "Weizhong" (BL 40) on morphology and expression of creatine kinase (CK) and interleukin-17 (IL-17) in rats with bupivacaine-induced multifidus muscle injury.
A total of 32 male SD rats were randomly divided into a control group, a model group, a Weizhong group and a Shenshu group, 8 rats in each one. The rats in the model group, Weizhong group and Shenshu group were treated with intramuscular injection of 0.5% bupivacaine to establish the model of multifidus muscle injury; the rats in the control group were injected with 0.9% sodium chloride solution. The rats in the Weizhong group and Shenshu group were treated with EA (2 Hz/10 Hz in frequency, 1~2 mA in intensity) at "Weizhong" (BL 40) and "Shenshu" (BL 23), 20 min per treatment. No treatment was given in the control group and model group. After 14-day treatment of EA, the inflammatory cell count, scar tissues area and muscle fiber cross sectional area of multifidus muscle were observed with HE and Masson staining method. The activity of CK and serum content of IL-17 were test with enzyme-linked immunosorbent assay (ELISA) method; the expression of IL-17 in multifidus muscle was measured with immunohistochcmical method.
After intervention, the inflammatory cell count and scar tissues area in the model group, Weizhong group and Shenshu group were higher than those in the control group (all <0.01), but the muscle fiber cross sectional area was significantly reduced (all <0.01); the inflammatory cell count and scar tissues area in the Weizhong group and Shenshu group were lower than those in the model group (all <0.01), and the muscle fiber cross sectional area was significantly increased (<0.01, <0.05). After intervention, the expression of IL-17 in multifidus muscle, serum content of IL-7 and activity of CK in the model group, Weizhong group and Shenshu group were higher than those in the control group (all <0.01); the expression of IL-17 in multifidus muscle, serum content of IL-7 and activity of CK in the Weizhong group and Shenshu group were lower than those in the model group (<0.01, <0.05); compared with the Shenshu group, the down-regulation of IL-17 was more obvisous in the Weizhong group (<0.01).
EA at "Weizhong" (BL 40) can down-regulate the overexpression of serum CK and IL-17, alleviate inflammation reaction and improve the repair of multifidus muscle.
观察电针“委中”（BL 40）对布比卡因诱导的大鼠多裂肌损伤后肌酸激酶（CK）及白细胞介素-17（IL-17）形态和表达的影响。
将32只雄性SD大鼠随机分为对照组、模型组、委中组和肾俞组，每组8只。模型组、委中组和肾俞组大鼠通过肌内注射0.5%布比卡因建立多裂肌损伤模型；对照组大鼠注射0.9%氯化钠溶液。委中组和肾俞组大鼠分别于“委中”（BL 40）和“肾俞”（BL 23）进行电针治疗（频率2 Hz/10 Hz，强度1~2 mA），每次治疗20分钟。对照组和模型组不做处理。电针治疗14天后，采用HE和Masson染色法观察多裂肌的炎性细胞计数、瘢痕组织面积及肌纤维横截面积。采用酶联免疫吸附测定（ELISA）法检测CK活性及血清IL-17含量；采用免疫组织化学方法检测多裂肌中IL-17的表达。
干预后，模型组、委中组和肾俞组的炎性细胞计数和瘢痕组织面积均高于对照组（均P<0.01），但肌纤维横截面积明显减小（均P<0.01）；委中组和肾俞组的炎性细胞计数和瘢痕组织面积均低于模型组（均P<0.01），且肌纤维横截面积明显增大（P<0.01，P<0.05）。干预后，模型组、委中组和肾俞组多裂肌中IL-17的表达、血清IL-7含量及CK活性均高于对照组（均P<0.01）；委中组和肾俞组多裂肌中IL-17的表达、血清IL-7含量及CK活性均低于模型组（P<0.01，P<0.05）；与肾俞组比较，委中组IL-17的下调更明显（P<0.01）。
电针“委中”（BL 40）可下调血清CK及IL-17的过表达，减轻炎症反应，促进多裂肌的修复。
