Mirisklavos A, Mottram P L, Dumble L J, Clunie G J
Int Arch Allergy Appl Immunol. 1986;79(2):215-9. doi: 10.1159/000233974.
The effect of Cyclosporine A (CsA) on T cell-mediated delayed-type hypersensitivity (DTH) reactions to murine alloantigens was analysed by transferring spleen cells from sensitised and suppressed mice into irradiated naive recipients. Selective removal of Thy1+, Ly1+ or Ly2+ cells prior to intravenous transfer of cells from alloantigen-sensitised mice showed that Ly1+ Thy1+ cells transferred sensitivity. Concomitant treatment of mice with alloantigen and CsA suppressed the DTH response to alloantigens, and spleen cells transferred from these suppressed mice abrogated the response of sensitised cells transferred at the same time. The suppressor cells were strain-restricted and antigen-specific with the surface phenotype Thy1+, Ly1-Ly2+.
通过将致敏和受抑制小鼠的脾细胞转移到经辐照的未致敏受体中，分析了环孢素A（CsA）对T细胞介导的针对小鼠同种异体抗原的迟发型超敏反应（DTH）的影响。在从同种异体抗原致敏小鼠静脉内转移细胞之前，选择性去除Thy1 +、Ly1 +或Ly2 +细胞表明，Ly1 + Thy1 +细胞传递了敏感性。用同种异体抗原和CsA同时处理小鼠可抑制对同种异体抗原的DTH反应，并且从这些受抑制小鼠转移的脾细胞消除了同时转移的致敏细胞的反应。抑制细胞具有品系限制性和抗原特异性，其表面表型为Thy1 +、Ly1 - Ly2 +。
