Department of Biochemistry, P.D. Patel Institute of Applied Sciences, Charotar University of Science & Technology, Changa; Department of IVF, Akanksha Hospital & Research Institute, A Unit of Sat Kaival Hospital Pvt. Ltd., Anand, India.
Department of IVF, Akanksha Hospital & Research Institute, A Unit of Sat Kaival Hospital Pvt. Ltd., Anand, India.
Indian J Med Res. 2017 Sep;146(3):341-345. doi: 10.4103/ijmr.IJMR_1702_15.
BACKGROUND & OBJECTIVES: Cumulus cell co-culture of embryo had been found to be beneficial for achieving better pregnancy and implantation rate (IR). The present study was aimed to evaluate efficiency of cumulus co-culture technique over simple culture of embryo in terms of pregnancy rate (PR) and IR in patients undergoing treatment for infertility using donor oocytes fertilized by intracytoplasmic sperm injection.
This was a quasi-experimental study between control and study groups. The primary endpoint was achievement of pregnancy. Control group included 508 women who underwent embryo development without cumulus cell co-culture and study group included 394 women who underwent embryo development with cumulus cell co-culture using donor's cumulus cells.
The present study demonstrated a significant increase in the IR (37.2 vs 24.2%, P<0.001) and in PR (45.7 vs 37.8%, P<0.05) in study group than in control group. The PR and IR were found to be higher in study group, among all groups of women, grouped on the basis of different indications for use of donor oocytes.
INTERPRETATION & CONCLUSIONS: Cumulus cell co-culture technique was found to be more effective than simple culture technique for embryo development in women undergoing treatment for infertility using donor oocytes fertilized by intracytoplasmic sperm injection.
已发现胚胎的卵丘细胞共培养有益于提高妊娠率和着床率（IR）。本研究旨在评估卵丘细胞共培养技术在使用卵胞浆内单精子注射受精的供体卵治疗不孕患者中对胚胎发育的妊娠率（PR）和 IR 的影响。
这是一项对照和研究组的准实验研究。主要终点是妊娠的实现。对照组包括 508 名未进行卵丘细胞共培养的胚胎发育患者，研究组包括 394 名使用供体卵丘细胞进行卵丘细胞共培养的胚胎发育患者。
本研究表明，与对照组相比，研究组的 IR（37.2%比 24.2%，P<0.001）和 PR（45.7%比 37.8%，P<0.05）显著增加。在所有因使用供体卵而接受治疗的不孕患者中，根据不同的适应证进行分组，研究组的 PR 和 IR 均较高。
与简单的胚胎培养技术相比，卵丘细胞共培养技术在使用卵胞浆内单精子注射受精的供体卵治疗不孕患者中更有效地促进胚胎发育。
